Electroacupuncture at ST36 Protects ICC Networks via mSCFKit-ETV1 Signaling in the Stomach of Diabetic Mice
Joint Authors
Tian, Lugao
Zhu, Beibei
Liu, Shi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-01-22
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Background.
Electroacupuncture (EA) at ST36 has been used to regulate gastric motility and effectively improve gastric emptying in diabetic patients.
Nevertheless, the specific mechanisms underlying the efficacy of this treatment remain unknown.
The aim of this study was to assess the variations of interstitial cells of Cajal (ICC) and explore the changes in mSCF/KIT-ETV1 signaling in the antrum and corpus of diabetic mice after treatment with EA.
Methods.
Male C57BL/6 mice were randomized into five groups: control group, diabetic group (DM), diabetic-plus-sham EA group (SEA), diabetic-plus-low-frequency EA group (LEA), and diabetic-plus-high-frequency EA group (HEA).
The expression levels of Ano1, c-Kit, and ETV1 were assessed by immunofluorescence in the antrum and corpus.
Western blotting and PCR methods were further used to evaluate c-Kit, mSCF, and ETV1 expression.
Results.
(1) c-Kit and Ano1 were obviously decreased in the DM group, but c-Kit reduced much more than Ano1.
(2) The mSCF, c-Kit, and ETV1 mRNA and protein levels were obviously decreased in the DM group in both the antrum and the corpus (P<0.01), but they were significantly elevated in the LEA and HEA groups (P<0.01).
Conclusions.
Ano1 is a reliable marker to detect ICC changes in diabetes; low- and high-frequency EA at acupoint ST36 can protect the networks of ICC possibly via normal activation of mSCF/KIT-ETV1 signaling.
American Psychological Association (APA)
Tian, Lugao& Zhu, Beibei& Liu, Shi. 2017. Electroacupuncture at ST36 Protects ICC Networks via mSCFKit-ETV1 Signaling in the Stomach of Diabetic Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1153613
Modern Language Association (MLA)
Tian, Lugao…[et al.]. Electroacupuncture at ST36 Protects ICC Networks via mSCFKit-ETV1 Signaling in the Stomach of Diabetic Mice. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-13.
https://search.emarefa.net/detail/BIM-1153613
American Medical Association (AMA)
Tian, Lugao& Zhu, Beibei& Liu, Shi. Electroacupuncture at ST36 Protects ICC Networks via mSCFKit-ETV1 Signaling in the Stomach of Diabetic Mice. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-13.
https://search.emarefa.net/detail/BIM-1153613
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153613