Study of Guanxinning Injection on Regulatory Mechanism of Bcl-2 and Bax by Liquid Nitrogen Freezing-Mediated Femoral Head Necrosis
Joint Authors
Xu, Xilin
Zhang, Xiaofeng
Lv, Hang
Li, Xiaodong
Zhang, Jie
Su, Hui
Liu, Peiran
Hu, Hai
Litscher, Daniela
Wang, Lu
Litscher, Gerhard
Sheng, Zemin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-05-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The objective of this study was to investigate the impact of femoral head perfusion by traditional Chinese medicine Guanxinning injection promoting blood circulation for removing blood stasis on the expression of Bcl-2 and Bax induced by liquid nitrogen freezing-mediated femoral head necrosis.
90 rabbits were randomized into three groups.
Normal control group was not subjected to any medication.
Saline and Guanxinning group were perfused with 0.9% saline and Guanxinning injection once every three days through the hip joint, respectively.
Six animals in each group were sacrificed at weeks 1, 3, 6, 9, and 12.
PCR and Western blot measured the expressions of Bcl-2 and Bax in the femoral head.
The bax expression in the Guanxinning group reduced at the third week significantly compared to the normal control group (P<0.01).
The Bcl-2 expression in the Guanxinning group increased substantially at the third week (P<0.05 or P<0.01).
Prolonged treatment elevated the expression of Bcl-2 in the Guanxinning group while that of Bax reduced remarkably (P<0.01).
Moreover, the ratio of Bcl-2 to Bax increased gradually in the Guanxinning group with prolonged drug administration.
Guanxinning injection can inhibit the cell apoptosis of femoral head necrosis through the treatment by femoral head perfusion.
American Psychological Association (APA)
Zhang, Xiaofeng& Xu, Xilin& Litscher, Gerhard& Sheng, Zemin& Wang, Lu& Litscher, Daniela…[et al.]. 2017. Study of Guanxinning Injection on Regulatory Mechanism of Bcl-2 and Bax by Liquid Nitrogen Freezing-Mediated Femoral Head Necrosis. Evidence-Based Complementary and Alternative Medicine،Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1153788
Modern Language Association (MLA)
Zhang, Xiaofeng…[et al.]. Study of Guanxinning Injection on Regulatory Mechanism of Bcl-2 and Bax by Liquid Nitrogen Freezing-Mediated Femoral Head Necrosis. Evidence-Based Complementary and Alternative Medicine No. 2017 (2017), pp.1-8.
https://search.emarefa.net/detail/BIM-1153788
American Medical Association (AMA)
Zhang, Xiaofeng& Xu, Xilin& Litscher, Gerhard& Sheng, Zemin& Wang, Lu& Litscher, Daniela…[et al.]. Study of Guanxinning Injection on Regulatory Mechanism of Bcl-2 and Bax by Liquid Nitrogen Freezing-Mediated Femoral Head Necrosis. Evidence-Based Complementary and Alternative Medicine. 2017. Vol. 2017, no. 2017, pp.1-8.
https://search.emarefa.net/detail/BIM-1153788
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153788