Clinical Significance and Prognostic Value of miR-28-5p in Colon Cancer
Joint Authors
Chen, Ying
Li, Ji-lin
Li, Ke-zhi
Xie, Ming-zhi
Tang, Yan-ping
Hu, Bangli
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-20
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
The association of miR-28-5p with colon cancer remains to be elucidated.
This study aimed to determine the clinical significance and prognostic value of miR-28-5p in colon cancer.
Methods.
We retrospectively analyzed the data of miR-28-5p in colon adenocarcinoma data from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO), and the data was divided into cancer group and normal group, respectively.
Forty colon cancer tissues and adjacent normal tissues were collected and tested by qRT-PCR methods.
The difference of the miR-28-5p expression between colon cancer and normal tissues was compared.
The clinical significance of miR-28-5p in colon cancer and the association with the survival were determined.
The predictive value of miR-28-5p in clinical features was determined using receiver operating characteristic curve.
The target genes of miR-28-5p were identified, and the functional of target genes was performed using bioinformatics analysis.
Results: The expression of miR-28-5p was increased in colon cancer tissues compared with normal controls (p=0.037).
The expression of miR-28-5p was significantly increased in tissues with distant metastases compared with that without distant metastases (p=0.026).
Patients with high expression of miR-28-5p have a shorter survival time than those with low expression (p=0.004).
Cox analysis showed that miR-28-5p was an independent predictor for the survival of patients (p=0.014).
Combination of miR-28-5p with TNM stage and clinical stage can improve the prognostic value for the patients (p<0.05).
miR-28-5p has a moderate predictive value in predicting the TNM stage and clinical stage (T stage: AUC=0.515; N stage: AUC=0.523, M stage: AUC=0.572; clinical stage: AUC=0.539).
711 potential target genes of miR-28-5p were screened; their function and pathways were identified.
Conclusions: This study demonstrated that miR-28-5p was increased in colon cancer and can be an independent indicator for the overall survival in patients with colon cancer.
American Psychological Association (APA)
Li, Ji-lin& Li, Ke-zhi& Xie, Ming-zhi& Tang, Yan-ping& Chen, Ying& Hu, Bangli. 2020. Clinical Significance and Prognostic Value of miR-28-5p in Colon Cancer. Disease Markers،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1153815
Modern Language Association (MLA)
Li, Ji-lin…[et al.]. Clinical Significance and Prognostic Value of miR-28-5p in Colon Cancer. Disease Markers No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1153815
American Medical Association (AMA)
Li, Ji-lin& Li, Ke-zhi& Xie, Ming-zhi& Tang, Yan-ping& Chen, Ying& Hu, Bangli. Clinical Significance and Prognostic Value of miR-28-5p in Colon Cancer. Disease Markers. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1153815
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1153815