Effects of Qi-Fang-Xi-Bi-Granules on Cartilage Morphology and Cebpα Promoter Methylation in Rats with Knee Osteoarthritis
Joint Authors
Qiu, HongFu
Xu, Hao
Wang, Xinxin
Shen, Peng
Wu, Tao
Gao, Xiang
Tang, De-Zhi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-01-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Objective.
To investigate the effects of Qi-Fang-Xi-Bi-Granules (QFXBGs) on cartilage morphology and methylation of C/ebpα (CCAAT/enhancer binding proteinα) at the promoter region.
Methods.
Knee osteoarthritis (KOA) modeling was performed in rats in accordance with Hulth’s method, and control group received sham operation.
Eight weeks after KOA modeling, the rats in the KOA modeling group were further divided into 6 groups.
Each group was given the appropriate drug.
After 8 weeks, half of the rats were used for Micro-CT scan, HE staining, ABH/OG staining, immunohistochemistry, and TUNNEL staining of the knee joint tissue, and the other half were used to examine C/ebpα promoter methylation.
Results.
The three dose groups of QFXBGs all showed lower degrees of surface fissures and flaking, thicker cartilage layer, and restored chondrocyte and subchondral bone morphology, compared with the KOA model group.
C/ebpα-22 promoter methylation levels in the high- and low-dose groups were significantly higher than that in the KOA modeling group (p<0.05), while C/ebpα-2 promoter methylation level in the medium-dose group was significantly higher than that in the KOA modeling group (p<0.05).
Conclusions.
QFXBGs may alleviate articular cartilage degeneration through promoting C/ebpα-2 or C/ebpα-22 methylation at specific promoter sites.
American Psychological Association (APA)
Wang, Xinxin& Shen, Peng& Tang, De-Zhi& Xu, Hao& Qiu, HongFu& Wu, Tao…[et al.]. 2018. Effects of Qi-Fang-Xi-Bi-Granules on Cartilage Morphology and Cebpα Promoter Methylation in Rats with Knee Osteoarthritis. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1154532
Modern Language Association (MLA)
Wang, Xinxin…[et al.]. Effects of Qi-Fang-Xi-Bi-Granules on Cartilage Morphology and Cebpα Promoter Methylation in Rats with Knee Osteoarthritis. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1154532
American Medical Association (AMA)
Wang, Xinxin& Shen, Peng& Tang, De-Zhi& Xu, Hao& Qiu, HongFu& Wu, Tao…[et al.]. Effects of Qi-Fang-Xi-Bi-Granules on Cartilage Morphology and Cebpα Promoter Methylation in Rats with Knee Osteoarthritis. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1154532
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154532