Establishment and Validation of an In Vitro Screening Method for Traditional Chinese Medicine-Induced Nephrotoxicity
Joint Authors
Zhu, Yan
Wang, Meng
Dong, Ran-Ran
Ma, Zhe
Cao, Xuexiao
Guo, Xiao
Ren, Xiaoliang
Shao, Rui
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-28
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Renal injury is among the adverse drug reactions (ADRs) caused by herbal medicine products (HMPs).
Traditional Chinese medicines (TCMs) have been practiced for over 2000 years in China and East Asia, and herbs are currently used worldwide for the treatment and prevention of chronic and acute disease.
Operetta high content analysis (HCA, PerkinElmer, Waltham, MA, USA), which is an in vitro, sensitive, reproducible, multiparametric screening method, was used to evaluate the cytotoxicity of HMPs in cultures of HEK293 human embryo kidney cells.
Cytotoxic results were validated by an animal-based subacute toxicity assay.
The renal safety of 18 active pharmaceutical agents from 13 TCM herbs with known nephrotoxic potential was evaluated in HEK293 human embryonic kidney cells.
A panel of five parameters, cell viability, nuclear area, nuclear roundness, mitochondrial mass, and mitochondrial membrane potential, was utilized to evaluate drug-induced renal mitochondrial and nuclear injury.
HCA can be a useful tool for preclinical screening and postclinical evaluation of HMPs.
The nephrotoxicity of diosbulbin B and other HMPs was evident at a concentration as low as 0.01 μM.
American Psychological Association (APA)
Ma, Zhe& Cao, Xuexiao& Guo, Xiao& Wang, Meng& Ren, Xiaoliang& Dong, Ran-Ran…[et al.]. 2018. Establishment and Validation of an In Vitro Screening Method for Traditional Chinese Medicine-Induced Nephrotoxicity. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1154600
Modern Language Association (MLA)
Ma, Zhe…[et al.]. Establishment and Validation of an In Vitro Screening Method for Traditional Chinese Medicine-Induced Nephrotoxicity. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-15.
https://search.emarefa.net/detail/BIM-1154600
American Medical Association (AMA)
Ma, Zhe& Cao, Xuexiao& Guo, Xiao& Wang, Meng& Ren, Xiaoliang& Dong, Ran-Ran…[et al.]. Establishment and Validation of an In Vitro Screening Method for Traditional Chinese Medicine-Induced Nephrotoxicity. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-15.
https://search.emarefa.net/detail/BIM-1154600
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154600