![](/images/graphics-bg.png)
The Synergy and Mode of Action of Cyperus rotundus L. Extract Plus Ampicillin against Ampicillin-Resistant Staphylococcus aureus
Joint Authors
Eumkeb, Griangsak
Cheypratub, Prairadda
Leeanansaksiri, Wilairat
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-03
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Cyperus rotundus L.
has been used for pharmaceutical applications including antibacterial infections.
Nevertheless, there is still no data regarding the mode of actions.
This study aimed to determine the antibacterial activity and mode of actions of Cyperus rotundus extract (CRE) against ampicillin-resistant Staphylococcus aureus (ARSA) which poses a serious problem for hospitalized patients.
The majority of chemical compounds of CRE were flavonoids and alkaloids.
The minimum inhibitory concentrations (MICs) for ampicillin and CRE against all ARSA strains were 64 μg/ml and 0.5 mg/ml, respectively.
Checkerboard assay revealed synergistic activity in the combination of ampicillin and CRE at the lowest fractional inhibitory concentration index (FICI) at 0.27.
The killing curve assay had confirmed the synergistic and bactericidal activity of the combination against ARSA.
Electron microscopic results showed that these ARSA cells treated with this combination caused peptidoglycan and cytoplasmic membrane (CM) damage and average cell areas significantly smaller than control.
Also, this combination caused an increase in CM permeability of ARSA.
CRE revealed the inhibitory activity against β-lactamase.
It is normally known that some drugs are derived from flavonoids or alkaloids.
So, this CRE proposes the potential to develop a novel adjunct phytopharmaceutical to ampicillin for the remedy of ARSA.
American Psychological Association (APA)
Cheypratub, Prairadda& Leeanansaksiri, Wilairat& Eumkeb, Griangsak. 2018. The Synergy and Mode of Action of Cyperus rotundus L. Extract Plus Ampicillin against Ampicillin-Resistant Staphylococcus aureus. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1154853
Modern Language Association (MLA)
Cheypratub, Prairadda…[et al.]. The Synergy and Mode of Action of Cyperus rotundus L. Extract Plus Ampicillin against Ampicillin-Resistant Staphylococcus aureus. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1154853
American Medical Association (AMA)
Cheypratub, Prairadda& Leeanansaksiri, Wilairat& Eumkeb, Griangsak. The Synergy and Mode of Action of Cyperus rotundus L. Extract Plus Ampicillin against Ampicillin-Resistant Staphylococcus aureus. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1154853
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154853