Modulation of Neuronal Activity on Intercalated Neurons of Amygdala Might Underlie Anxiolytic Activity of a Standardized Extract of Centella asiatica ECa233
Joint Authors
Wanasuntronwong, Aree
Wanakhachornkrai, Oraphan
Phongphanphanee, Penphimon
Isa, Tadashi
Tantisira, Boonyong
Tantisira, Mayuree H.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
GABAergic intercalated neurons of amygdala (ITCs) have recently been shown to be important in the suppression of fear-like behavior.
Effects of ECa233 (a standardized extract of Centella asiatica), previously demonstrated anxiolytic activity, were then investigated on ITCs.
Cluster of GABAergic neurons expressing fluorescence of GFP was identified in GAD67-GFP knock-in mice.
We found that neurons of medial paracapsular ITC were GABAergic neurons exhibiting certain intrinsic electrophysiological properties similar to those demonstrated by ITC neurons at the same location in C57BL/6J mice.
Therefore, we conducted experiments in both C57BL/6J mice and GAD67-GFP knock-in mice.
Excitatory postsynaptic currents (EPSCs) were evoked by stimulation of the external capsule during the whole cell patch-clamp recordings from ITC neurons in brain slices.
ECa233 was found to increase the EPSC peak amplitude in the ITC neurons by about 120%.
The EPSCs in ITC neurons were completely abolished by the application of an AMPA receptor antagonist.
Morphological assessment of the ITC neurons with biocytin demonstrated that most axons of the recorded neurons innervated the central nucleus of the amygdala (CeA).
Therefore, it is highly likely that anxiolytic activity of ECa233 was mediated by increasing activation, via AMPA receptors, of excitatory synaptic input to the GABAergic ITC leading to depression of CeA neurons.
American Psychological Association (APA)
Wanasuntronwong, Aree& Wanakhachornkrai, Oraphan& Phongphanphanee, Penphimon& Isa, Tadashi& Tantisira, Boonyong& Tantisira, Mayuree H.. 2018. Modulation of Neuronal Activity on Intercalated Neurons of Amygdala Might Underlie Anxiolytic Activity of a Standardized Extract of Centella asiatica ECa233. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1154993
Modern Language Association (MLA)
Wanasuntronwong, Aree…[et al.]. Modulation of Neuronal Activity on Intercalated Neurons of Amygdala Might Underlie Anxiolytic Activity of a Standardized Extract of Centella asiatica ECa233. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-8.
https://search.emarefa.net/detail/BIM-1154993
American Medical Association (AMA)
Wanasuntronwong, Aree& Wanakhachornkrai, Oraphan& Phongphanphanee, Penphimon& Isa, Tadashi& Tantisira, Boonyong& Tantisira, Mayuree H.. Modulation of Neuronal Activity on Intercalated Neurons of Amygdala Might Underlie Anxiolytic Activity of a Standardized Extract of Centella asiatica ECa233. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-8.
https://search.emarefa.net/detail/BIM-1154993
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1154993