Study on the Mechanism of Sarsasapogenin in Treating Precocious Puberty by Regulating the HPG Axis
Joint Authors
Chang, Hong-sheng
Hu, Kaili
Sun, Wenyan
Li, Yu
Zhang, Bo
Zhang, Meng
Guo, Chunyan
Wang, Xiaoling
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The present study aims to investigate the effects and mechanisms of sarsasapogenin resistance to precocious puberty.
Female Sprague Dawley rats were divided into a normal (N) group, model (M) group, leuprolide (L) group, and sarsasapogenin (Sar) group.
Rats at 5 days of age were given a single subcutaneous injection of 300 micrograms of danazol to establish the precocious puberty model.
After 10 days of modeling, drug intervention was started.
The development of the uterus and ovary was observed by hematoxylin and eosin (HE) staining.
The levels of the serum luteinizing hormone (LH), follicle-stimulating hormone (FSH), and estradiol (E2) were determined by radioimmunoassay.
Also, the expressions of the hypothalamic gonadotropin releasing hormone (GnRH), Kiss-1, G protein-coupled receptor 54 (GPR54), and pituitary gonadotropin releasing hormone receptor (GnRH-R) were detected by RT-PCR.
The results showed that compared with the model group, sarsasapogenin could significantly delay the opening time of vaginal, decreased uterine and ovarian coefficients, and reduced uterine wall thickness.
Moreover, it can significantly downregulate the levels of serum hormones and reduce the expression of GnRH, GnRH-R, and kiss-1.
In summary, our results indicate that sarsasapogenin can regulate the HPG axis through the kiss-1/GPR54 system for therapeutic precocious puberty.
American Psychological Association (APA)
Hu, Kaili& Sun, Wenyan& Li, Yu& Zhang, Bo& Zhang, Meng& Guo, Chunyan…[et al.]. 2020. Study on the Mechanism of Sarsasapogenin in Treating Precocious Puberty by Regulating the HPG Axis. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1155032
Modern Language Association (MLA)
Hu, Kaili…[et al.]. Study on the Mechanism of Sarsasapogenin in Treating Precocious Puberty by Regulating the HPG Axis. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1155032
American Medical Association (AMA)
Hu, Kaili& Sun, Wenyan& Li, Yu& Zhang, Bo& Zhang, Meng& Guo, Chunyan…[et al.]. Study on the Mechanism of Sarsasapogenin in Treating Precocious Puberty by Regulating the HPG Axis. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1155032
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155032