(+)-Usnic Acid Inhibits Migration of c-KIT Positive Cells in Human Colorectal Cancer
Joint Authors
Wu, Wei
Hou, Bing
Tang, Changli
Liu, Fucheng
Yang, Jie
Pan, Tao
Si, Ke
Lu, Deyun
Wang, Xiaoxiang
Wang, Jing
Xiong, Xing
Liu, Ji
Xie, Chunguang
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-12
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Inhibition of tumor cell migration is a treatment strategy for patients with colorectal cancer (CRC).
SCF-dependent activation of c-KIT is responsible for migration of c-KIT positive [c-KIT(+)] cells of CRC.
Drug resistance to Imatinib Mesylate (c-KIT inhibitor) has emerged.
Inhibition of mTOR can induce autophagic degradation of c-KIT.
(+)-usnic acid [(+)-UA], isolated from lichens, has two major functions including induction of proton shuttle and targeting inhibition of mTOR.
To reduce hepatotoxicity, the treatment concentration of (+)-UA should be lower than 10 μM.
HCT116 cells and LS174 cells were employed to investigate the inhibiting effect of (+)-UA (<10 μM) on SCF-mediated migration of c-KIT(+) CRC cells.
HCT116 cells were employed to investigate the molecular mechanisms.
The results indicated that firstly, 8 μM (+)-UA decreased ATP content via uncoupling; secondly, 8 μM (+)-UA induced mTOR inhibition, thereby mediated activation suppression of PKC-A, and induced the autophagy of the completed autophagic flux that resulted in the autophagic degradation and transcriptional inhibition of c-KIT and the increase in LDH release; ultimately, 8 μM (+)-UA inhibited SCF-mediated migration of CRC c-KIT(+) cells.
Taken together, 8 μM could be determined as the effective concentration for (+)-UA to inhibit SCF-mediated migration of CRC c-KIT(+) cells.
American Psychological Association (APA)
Wu, Wei& Hou, Bing& Tang, Changli& Liu, Fucheng& Yang, Jie& Pan, Tao…[et al.]. 2018. (+)-Usnic Acid Inhibits Migration of c-KIT Positive Cells in Human Colorectal Cancer. Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1155421
Modern Language Association (MLA)
Wu, Wei…[et al.]. (+)-Usnic Acid Inhibits Migration of c-KIT Positive Cells in Human Colorectal Cancer. Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-13.
https://search.emarefa.net/detail/BIM-1155421
American Medical Association (AMA)
Wu, Wei& Hou, Bing& Tang, Changli& Liu, Fucheng& Yang, Jie& Pan, Tao…[et al.]. (+)-Usnic Acid Inhibits Migration of c-KIT Positive Cells in Human Colorectal Cancer. Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-13.
https://search.emarefa.net/detail/BIM-1155421
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155421