Identification of Active Compounds of Mahuang Fuzi Xixin Decoction and Their Mechanisms of Action by LC-MSMS and Network Pharmacology
Joint Authors
Tang, Qingfa
Liang, Xiao
Liu, Chang-Shun
Xia, Ting
Tan, Xiao-Mei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-25
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
The decoction is an important dosage form of traditional Chinese medicine (TCM) administration.
The Mahuang Fuzi Xixin decoction (MFXD) is widely used to treat allergic rhinitis (AR) in China.
However, its active compounds and therapeutic mechanisms are unclear.
The aim of this study was to establish an integrative method to identify the bioactive compounds and reveal the mechanisms of action of MFXD.
LC-MS/MS was used to identify the compounds in MFXD, followed by screening for oral bioavailability.
TCMSP, BindingDB, STRING, DAVID, and KEGG databases and algorithms were used to gather information.
Cytoscape was used to visualize the networks.
Twenty-four bioactive compounds were identified, and thirty-seven predicted targets of these compounds were associated with AR.
DAVID analysis suggested that these compounds exert their therapeutic effects by modulating the Fc epsilon RI, B-cell receptor, Toll-like receptor, TNF, NF-κB, and T-cell receptor signaling pathways.
The PI3K/AKT and cAMP signaling pathways were also implicated.
Ten of the identified compounds, quercetin, pseudoephedrine, ephedrine, β-asarone, methylephedrine, α-linolenic acid, cathine, ferulic acid, nardosinone, and higenamine, seemed to account for most of the beneficial effects of MFXD in AR.
This study showed that LC-MS/MS followed by network pharmacology analysis is useful to elucidate the complex mechanisms of action of TCM formulas.
American Psychological Association (APA)
Liang, Xiao& Liu, Chang-Shun& Xia, Ting& Tang, Qingfa& Tan, Xiao-Mei. 2020. Identification of Active Compounds of Mahuang Fuzi Xixin Decoction and Their Mechanisms of Action by LC-MSMS and Network Pharmacology. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1155934
Modern Language Association (MLA)
Liang, Xiao…[et al.]. Identification of Active Compounds of Mahuang Fuzi Xixin Decoction and Their Mechanisms of Action by LC-MSMS and Network Pharmacology. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-11.
https://search.emarefa.net/detail/BIM-1155934
American Medical Association (AMA)
Liang, Xiao& Liu, Chang-Shun& Xia, Ting& Tang, Qingfa& Tan, Xiao-Mei. Identification of Active Compounds of Mahuang Fuzi Xixin Decoction and Their Mechanisms of Action by LC-MSMS and Network Pharmacology. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-11.
https://search.emarefa.net/detail/BIM-1155934
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155934