Genotoxic and Chemopreventive Effects of Vochysia divergens Leaves (Pantanal, Brazil)
Joint Authors
de Oliveira, Pollyanna Francielli
Mendes, Suzana Amorim
Acésio, Nathália Oliveira
Filho, Luis Claudio Kellner
Pimenta, Leticia Pereira
Siqueira, Kátia Aparecida
Soares, Marcos Antônio
Januário, Ana Helena
Tavares, Denise Crispim
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-19
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The medicinal plant Vochysia divergens is a colonizing tree species of the Pantanal, a unique and little explored wetland region in Brazil.
This species is used in folk medicine as syrups and teas to treat respiratory infections, digestive disorders, asthma, scarring, and skin diseases.
The objectives of this study were to evaluate the antioxidant, cytotoxic, and genotoxic potential of the ethanolic extract of Vochysia divergens leaves (VdE), as well as the influence of VdE and its major component (the flavone 3′,5-dimethoxy luteolin-7-O-β-glucopyranoside; 3′5 DL) on MMS-induced genotoxicity.
The extract significantly reduced the viability of V79 cells in the colorimetric XTT assay at concentrations ≥ 39 μg/mL.
A significant increase in micronucleus frequencies was observed in V79 cell cultures treated with VdE concentrations of 160 and 320 μg/mL.
However, animals treated with the tested doses of VdE (500, 1000, and 2000 mg/kg b.w.) exhibited frequencies that did not differ significantly from those of the negative control group, indicating the absence of genotoxicity.
The results also showed that VdE was effective in reducing MMS-induced genotoxicity at concentrations of 20, 40, and 80 μg/mL in the in vitro test system and at a dose of 15 mg/kg b.w.
in the in vivo test system.
Its major component 3′5 DL exerted no protective effect, suggesting that it is not responsible for the effect of the extract.
The results of the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay showed that VdE was able to scavenge 92.6% of free radicals.
In conclusion, the results suggest that the protective effect of VdE may be related, at least in part, to the antioxidant activity of its chemical constituents.
American Psychological Association (APA)
de Oliveira, Pollyanna Francielli& Mendes, Suzana Amorim& Acésio, Nathália Oliveira& Filho, Luis Claudio Kellner& Pimenta, Leticia Pereira& Siqueira, Kátia Aparecida…[et al.]. 2018. Genotoxic and Chemopreventive Effects of Vochysia divergens Leaves (Pantanal, Brazil). Evidence-Based Complementary and Alternative Medicine،Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1155960
Modern Language Association (MLA)
de Oliveira, Pollyanna Francielli…[et al.]. Genotoxic and Chemopreventive Effects of Vochysia divergens Leaves (Pantanal, Brazil). Evidence-Based Complementary and Alternative Medicine No. 2018 (2018), pp.1-7.
https://search.emarefa.net/detail/BIM-1155960
American Medical Association (AMA)
de Oliveira, Pollyanna Francielli& Mendes, Suzana Amorim& Acésio, Nathália Oliveira& Filho, Luis Claudio Kellner& Pimenta, Leticia Pereira& Siqueira, Kátia Aparecida…[et al.]. Genotoxic and Chemopreventive Effects of Vochysia divergens Leaves (Pantanal, Brazil). Evidence-Based Complementary and Alternative Medicine. 2018. Vol. 2018, no. 2018, pp.1-7.
https://search.emarefa.net/detail/BIM-1155960
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155960