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Antimicrobial, Cytotoxic, and Antioxidant Potential of a Novel Flavone “6,7,4′-Trimethyl Flavone” Isolated from Wulfenia amherstiana
Joint Authors
Kakar, Maria
Amin, Muhammad Usman
Alghamdi, Saad
Sahibzada, Muhammad Umar Khayam
Ahmad, Nisar
Ullah, Naseem
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-24
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Wulfenia amherstiana belongs to the Scrophulariaceae family and various plants of this family are known for their biological activities.
The present study was focused on the isolation of bioactive compounds including a novel flavone 6,7,4′-trimethyl flavone (TMF) along with three known flavonoids such as quercetin, rutin, and a steroid β-sitosterol which were isolated from the ethanolic extract of W.
amherstiana (Himalayan Wulfenia) through column chromatography and purified by using HPLC.
Their structures were identified and elucidated through electron ionization mass spectroscopy (EIMS), 1DNMR (1H-NMR and 13C-NMR), and 2DNMR (COSY, HMQC, and HMBC) spectroscopy.
The antimicrobial activities of this novel compound were evaluated through agar well diffusion method, while antioxidant and cytotoxic activities were assessed through 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging assay and brine shrimp lethality assay, respectively.
The NMR data revealed that TMF is a novel compound.
TMF showed potential antibacterial and antifungal activities against Staphylococcus aureus (MIC = 128 μg/ml) and Candida albicans (MIC = 128 μg/ml).
The cytotoxic potential of TMF was determined from brine shrimp lethality assay with LD50 of 127.01 μg/ml.
The free-radical scavenging potential of TMF at various concentrations implicated its strong antioxidant activity in vitro.
The results revealed that TMF demonstrated substantial antimicrobial activity against S.
aureus and C.
albicans, strong antioxidant activity, and moderately cytotoxic activity.
American Psychological Association (APA)
Kakar, Maria& Amin, Muhammad Usman& Alghamdi, Saad& Sahibzada, Muhammad Umar Khayam& Ahmad, Nisar& Ullah, Naseem. 2020. Antimicrobial, Cytotoxic, and Antioxidant Potential of a Novel Flavone “6,7,4′-Trimethyl Flavone” Isolated from Wulfenia amherstiana. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1155984
Modern Language Association (MLA)
Kakar, Maria…[et al.]. Antimicrobial, Cytotoxic, and Antioxidant Potential of a Novel Flavone “6,7,4′-Trimethyl Flavone” Isolated from Wulfenia amherstiana. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1155984
American Medical Association (AMA)
Kakar, Maria& Amin, Muhammad Usman& Alghamdi, Saad& Sahibzada, Muhammad Umar Khayam& Ahmad, Nisar& Ullah, Naseem. Antimicrobial, Cytotoxic, and Antioxidant Potential of a Novel Flavone “6,7,4′-Trimethyl Flavone” Isolated from Wulfenia amherstiana. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1155984
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1155984