Simultaneous Quantification of Brigatinib and Brigatinib-Analog in Rat Plasma and Brain Homogenate by LC-MSMS: Application to Comparative Pharmacokinetic and Brain Distribution Studies
Joint Authors
Li, Bo
Chen, Su-Hong
Lei, Shan-Shan
Lu, Min
Jin, Lei
Zheng, Maoen
Sun, Peilu
Xiong, Shan
Source
International Journal of Analytical Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-12-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Brigatinib and brigatinib-analog are potent and selective ALK inhibitors with the similar structure.
A simple and sensitive high-performance liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for simultaneous determination of brigatinib and brigatinib-analog in rat plasma and brain homogenate was developed and validated.
Chromatographic separation was carried out on an ODS column with acetonitrile and 0.1% formic acid in water as the mobile phase with gradient elution at a flow rate of 0.5 mL/min.
Detections were performed using a TSQ Quantum Ultra mass spectrometric detector with electrospray ionization (ESI) interface, which was operated in the positive ion mode.
A simple protein precipitation preparation process was used.
The lower limits of quantification (LLOQs) were 1.0 ng/mL and 0.5 ng/mL for analytes in rat plasma and brain homogenate, respectively.
The intrabatch and interbatch precision and accuracy of brigatinib and brigatinib-analog were well within the acceptable limits of variation.
The simple and sensitive LC-MS/MS method was successfully applied to the pharmacokinetic and brain distribution studies following a single oral administration of brigatinib and brigatinib-analog to rats.
The above studies would lay a good foundation for the further applications of brigatinib and brigatinib-analog.
American Psychological Association (APA)
Li, Bo& Lu, Min& Jin, Lei& Zheng, Maoen& Sun, Peilu& Lei, Shan-Shan…[et al.]. 2019. Simultaneous Quantification of Brigatinib and Brigatinib-Analog in Rat Plasma and Brain Homogenate by LC-MSMS: Application to Comparative Pharmacokinetic and Brain Distribution Studies. International Journal of Analytical Chemistry،Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1156003
Modern Language Association (MLA)
Li, Bo…[et al.]. Simultaneous Quantification of Brigatinib and Brigatinib-Analog in Rat Plasma and Brain Homogenate by LC-MSMS: Application to Comparative Pharmacokinetic and Brain Distribution Studies. International Journal of Analytical Chemistry No. 2019 (2019), pp.1-10.
https://search.emarefa.net/detail/BIM-1156003
American Medical Association (AMA)
Li, Bo& Lu, Min& Jin, Lei& Zheng, Maoen& Sun, Peilu& Lei, Shan-Shan…[et al.]. Simultaneous Quantification of Brigatinib and Brigatinib-Analog in Rat Plasma and Brain Homogenate by LC-MSMS: Application to Comparative Pharmacokinetic and Brain Distribution Studies. International Journal of Analytical Chemistry. 2019. Vol. 2019, no. 2019, pp.1-10.
https://search.emarefa.net/detail/BIM-1156003
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156003