Whole-Exome Sequencing-Based Mutational Profiling of Hepatitis B Virus-Related Early-Stage Hepatocellular Carcinoma
Joint Authors
Hu, Jin-Wu
Sun, Qi-Man
Fan, Jia
Zhan, Hao
Jiang, Jiahao
Ke, Aiwu
Hu, Zhiqiang
Zhu, Kai
Luo, Chubin
Ren, Ning
Zhou, Jian
Huang, Xiaowu
Source
Gastroenterology Research and Practice
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-29
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
Hepatocellular carcinoma (HCC) ranks as the third leading cause of cancer-related mortality in China with increasing incidence.
This study is designed to explore early genetic changes implicated in HCC tumorigenesis and progression by whole-exome sequencing.
Methods.
We firstly sequenced the whole exomes of 5 paired hepatitis B virus-related early-stage HCC and peripheral blood samples, followed by gene ontological analysis and pathway analysis of the single-nucleotide variants discovered.
Then, the mutations of high frequency were further confirmed by Sanger sequencing.
Results.
We identified a mutational signature of dominant T:A>A:T transversion in early HCC and significantly enriched pathways including ECM-receptor interaction, axon guidance, and focal adhesion and enriched biological processes containing cell adhesion, axon guidance, and regulation of pH.
Eight genes, including MUC16, UNC79, USH2A, DNAH17, PTPN13, TENM4, PCLO, and PDE1C, were frequently mutated.
Conclusions.
This study reveals a mutational profile and a distinct mutation signature of T:A>A:T transversion in early-stage HCC with HBV infection, which will enrich our understanding of genetic characteristics of the early-stage HCC.
American Psychological Association (APA)
Zhan, Hao& Jiang, Jiahao& Sun, Qi-Man& Ke, Aiwu& Hu, Jin-Wu& Hu, Zhiqiang…[et al.]. 2017. Whole-Exome Sequencing-Based Mutational Profiling of Hepatitis B Virus-Related Early-Stage Hepatocellular Carcinoma. Gastroenterology Research and Practice،Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1156220
Modern Language Association (MLA)
Zhan, Hao…[et al.]. Whole-Exome Sequencing-Based Mutational Profiling of Hepatitis B Virus-Related Early-Stage Hepatocellular Carcinoma. Gastroenterology Research and Practice No. 2017 (2017), pp.1-7.
https://search.emarefa.net/detail/BIM-1156220
American Medical Association (AMA)
Zhan, Hao& Jiang, Jiahao& Sun, Qi-Man& Ke, Aiwu& Hu, Jin-Wu& Hu, Zhiqiang…[et al.]. Whole-Exome Sequencing-Based Mutational Profiling of Hepatitis B Virus-Related Early-Stage Hepatocellular Carcinoma. Gastroenterology Research and Practice. 2017. Vol. 2017, no. 2017, pp.1-7.
https://search.emarefa.net/detail/BIM-1156220
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156220