T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity
Joint Authors
Principi, Mariabeatrice
Losurdo, Giuseppe
Ierardi, Enzo
Di Leo, Alfredo
Iannone, Andrea
Piscitelli, Domenico
Pezzuto, Federica
Fortarezza, Francesco
Covelli, Claudia
Marra, Antonella
Amoruso, Annacinzia
Source
Gastroenterology Research and Practice
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-12-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Background and Aims.
Nonceliac gluten sensitivity (NCGS) is a gluten-related emerging condition.
Since few data about NCGS histopathology is available, we assessed the markers of lymphocyte and innate immunity activation.
Materials and Methods.
We retrieved duodenal biopsy samples of patients with NCGS diagnosis according to the Salerno criteria.
We selected specimens of positive (seropositive celiac disease/Marsh 1-2 stage) and negative (normal microscopic picture) controls.
Immunohistochemistry for CD3 (intraepithelial lymphocytes-IELs), CD4 (T helper lymphocytes), CD8 (T cytotoxic lymphocytes), and CD1a/CD117 (Langerhans/mast cells) was performed.
ANOVA plus Bonferroni’s tests were used for statistical analysis.
Results.
Twenty NCGS, 16 celiac disease, and 16 negative controls were selected.
CD3 in NCGS were higher than negative controls and lower than celiac disease (18.5 ± 6.4, 11.9 ± 2.8, and 40.8 ± 8.1 IELs/100 enterocytes; p<0.001).
CD4 were lower in NCGS than controls and celiac disease (31.0 ± 22.1, 72.5 ± 29.5, and 103.7 ± 15.7 cells/mm2; p<0.001).
CD8 in NCGS were similar to negative controls, but lower than celiac disease (14.0 ± 7.4 and 34.0 ± 7.1 IELs/100 enterocytes, p<0.001).
CD117 were higher in NCGS than celiac disease and negative controls (145.8 ± 49.9, 121.3 ± 13.1, and 113.5 ± 23.4 cells/mm2; p=0.009).
Conclusions.
The combination of CD4 and CD117, as well as IEL characterization, may be useful to support a clinical diagnosis of NCGS.
American Psychological Association (APA)
Losurdo, Giuseppe& Piscitelli, Domenico& Pezzuto, Federica& Fortarezza, Francesco& Covelli, Claudia& Marra, Antonella…[et al.]. 2017. T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity. Gastroenterology Research and Practice،Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1156436
Modern Language Association (MLA)
Losurdo, Giuseppe…[et al.]. T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity. Gastroenterology Research and Practice No. 2017 (2017), pp.1-9.
https://search.emarefa.net/detail/BIM-1156436
American Medical Association (AMA)
Losurdo, Giuseppe& Piscitelli, Domenico& Pezzuto, Federica& Fortarezza, Francesco& Covelli, Claudia& Marra, Antonella…[et al.]. T Helper Lymphocyte and Mast Cell Immunohistochemical Pattern in Nonceliac Gluten Sensitivity. Gastroenterology Research and Practice. 2017. Vol. 2017, no. 2017, pp.1-9.
https://search.emarefa.net/detail/BIM-1156436
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156436