The Protective Effect of Different Polar Solvent Extracts of Er Miao San on Rats with Adjuvant Arthritis
Joint Authors
Zhang, Wei
Zhang, Qiying
Xuan, Zihua
Liang, Juan
Yang, Dongping
Ding, Meihuizi
Zhu, Housheng
Su, Bo
Dai, Xing
Jia, Xiaoyi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-20
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Objective.
The aim of this study was to evaluate the antiarthritic effects of different polar solvent extracts of Er Miao San (EMS) on model rats with adjuvant arthritis (AA) and screen the effective pats of EMS in the treatment of arthritis.
Methods.
Four different polar solvent extracts of EMS such as petroleum ether (PE), methylene chloride (CH2Cl2), ethyl acetate (EtOAc), and n-butanol (n-BuOH) were extracted and separated by different solvent polar extraction.
Different polar parts of the aqueous extract of EMS were orally administered for 14 days to AA rats.
Progression of clinical signs such as edema of paws and polyarthritis index was measured.
The ankle joint changes were observed by pathological sections.
ELISA was used to measure cytokines in the serum according to the manufacturer’s instructions.
UHPLC measured the effective parts of EMS.
Results.
Administration of EtOAc and CH2Cl2 parts remarkably inhibited the paw swelling, decreased the index of arthritis, decreased the body weight loss, and improved the changes of histopathology.
Furthermore, the concentrations of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) were significantly lower, while the anti-inflammatory cytokine (IL-10) was remarkably higher compared with that in the model group.
And the result of UHPLC analysis indicated that the effective parts of EMS contain berberine and atractylodin.
Conclusions.
EtOAc and CH2Cl2 are the effective parts of EMS that can improve arthritis.
In particular, berberine and atractylodin may be responsible for the antiarthritic activity of EMS.
This research provided pharmacological and chemical foundation for the application of EMS in treating rheumatoid arthritis (RA).
American Psychological Association (APA)
Zhang, Wei& Zhang, Qiying& Xuan, Zihua& Liang, Juan& Yang, Dongping& Ding, Meihuizi…[et al.]. 2020. The Protective Effect of Different Polar Solvent Extracts of Er Miao San on Rats with Adjuvant Arthritis. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1156516
Modern Language Association (MLA)
Zhang, Wei…[et al.]. The Protective Effect of Different Polar Solvent Extracts of Er Miao San on Rats with Adjuvant Arthritis. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-8.
https://search.emarefa.net/detail/BIM-1156516
American Medical Association (AMA)
Zhang, Wei& Zhang, Qiying& Xuan, Zihua& Liang, Juan& Yang, Dongping& Ding, Meihuizi…[et al.]. The Protective Effect of Different Polar Solvent Extracts of Er Miao San on Rats with Adjuvant Arthritis. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-8.
https://search.emarefa.net/detail/BIM-1156516
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1156516