Extraction, Characterization, Antioxidant, and Immunostimulatory Activities of Polysaccharides from Hedyotis corymbosa

Abstract EN

In this study, optimization of enzyme-assisted extraction, purification, characterization, and its bioactivities of polysaccharides from Hedyotis corymbosa (HCP) was investigated.

It was found that the optimum extraction conditions were 3% of enzyme concentration (X 1), 30 of liquid-to-solid ratio (X 2), 56°C of extraction temperature (X 3), 200W of ultrasonic power (X 4), 10 min of extraction time (X 5), and 5 of pH value (X 6).

Under optimum conditions, the experimental yield (4.10 ± 0.16%) was closed to the predicted value (4.02%).

The crude HCP was further purified using DEAE-52 and Sephadex G-150 gel column, and a major polysaccharide fraction from HCP, designed as HCP-1a with molecular weight of 33.9 kDa, was obtained.

The HCP and HCP-1a were characterized by chemical analysis, FT-IR, and HPLC.

For antioxidant activities in vitro, HCP possessed strong hydroxyl radical scavenging, DPPH radical scavenging, and Fe2+ chelating activities.

In subsequent immunostimulatory studies, significantly decreased NO, IL-1β, and TNF-α concentrations were observed in both of HCP and HCP-1a treated RAW264.7 cells.

Therefore, this study may indicate some insights into the application of polysaccharides from Hedyotis corymbosa as potential natural antioxidants and immunostimulants.