Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry
Joint Authors
Galvez, Julio
Bribi, Noureddine
Gómez-Caravaca, Ana María
Segura-Carretero, Antonio
Contreras, María del Mar
Source
International Journal of Analytical Chemistry
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-28
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Two analytical platforms, gas chromatography (GC) coupled to quadrupole-time-of-flight (QTOF) mass spectrometry (MS) and reversed-phase ultrahigh performance liquid chromatography (UHPLC) coupled to diode array (DAD) and QTOF detection, were applied in order to study the alkaloid profile of Fumaria capreolata.
The use of these mass analyzers enabled tentatively identifying the alkaloids by matching their accurate mass signals and suggested molecular formulae with those previously reported in libraries and databases.
Moreover, the proposed structures were corroborated by studying their fragmentation pattern obtained by both platforms.
In this way, 8 and 26 isoquinoline alkaloids were characterized using GC-QTOF-MS and RP-UHPLC-DAD-QTOF-MS, respectively, and they belonged to the following subclasses: protoberberine, protopine, aporphine, benzophenanthridine, spirobenzylisoquinoline, morphinandienone, and benzylisoquinoline.
Moreover, the latter analytical method was selected to determine at 280 nm the concentration of protopine (9.6 ± 0.7 mg/g), a potential active compound of the extract.
In conclusion, although GC-MS has been commonly used for the analysis of this type of phytochemicals, RP-UHPLC-DAD-QTOF-MS provided essential complementary information.
This analytical method can be applied for the quality control of phytopharmaceuticals containing Fumaria extracts currently found in the market.
American Psychological Association (APA)
Contreras, María del Mar& Bribi, Noureddine& Gómez-Caravaca, Ana María& Galvez, Julio& Segura-Carretero, Antonio. 2017. Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry. International Journal of Analytical Chemistry،Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1157575
Modern Language Association (MLA)
Contreras, María del Mar…[et al.]. Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry. International Journal of Analytical Chemistry No. 2017 (2017), pp.1-16.
https://search.emarefa.net/detail/BIM-1157575
American Medical Association (AMA)
Contreras, María del Mar& Bribi, Noureddine& Gómez-Caravaca, Ana María& Galvez, Julio& Segura-Carretero, Antonio. Alkaloids Profiling of Fumaria capreolata by Analytical Platforms Based on the Hyphenation of Gas Chromatography and Liquid Chromatography with Quadrupole-Time-of-Flight Mass Spectrometry. International Journal of Analytical Chemistry. 2017. Vol. 2017, no. 2017, pp.1-16.
https://search.emarefa.net/detail/BIM-1157575
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157575