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Guizhi Fuling Capsule Exhibits Antidysmenorrhea Activity by Inhibition of Cyclooxygenase Activity
Joint Authors
Xiao, Wei
Zheng, Weiran
Li, Meng
Wang, Yongxiang
Lv, Baojie
Zhang, Xinzhuang
Chen, Lilan
Zhu, Kejin
Wang, Zhenzhong
Li, Baoxin
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-05-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Guizhi Fuling capsule (GZFLc) is a modern preparation from traditional Chinese Medicine.
Guizhi Fuling was first prescribed by Zhang Zhongjing almost two thousand years ago for the treatment of primary dysmenorrhea.
It has also been used to treat uterine fibroids, dysfunctional uterine bleeding, and endometriosis.
Although effective against dysmenorrhea clinically, there are limited information on the mechanism of its action.
The major components responsible for the activity are not well defined.
The aim of this study has been to elucidate a mechanism that may facilitate the development of a bioactivity-based assay for quality control during drug formulation and manufacturing.
Using an oxytocin-induced mouse dysmenorrhea model, we showed that oral administration of GZFLc at 150 and 300 mg/kg, dosages relevant to clinic usages, significantly suppressed oxytocin-induced writhing response.
The antidysmenorrhea effect was also demonstrated by a rotarod assay.
We showed that GZFLc treatment significantly prolonged the hanging time of mice on the rotating rod.
Histological studies showed that GZFLc treatment reduced lamina propria edema, while no effect on COX2 expression was detected.
GZFLc instead exhibited direct inhibitory effect against COX2, a critical enzyme that catalyzes arachidonic acid conversion to prostaglandins.
By HPLC profiling, we showed that paeoniflorin, paeonol, and cinnamaldehyde are the major components from the corresponding plants.
At 5 and 10 mg/kg, both paeoniflorin and paeonol were active against induced dysmenorrhea.
The study not only links GZFLc antidysmenorrhea activity to COX2 inhibition but also uncovers a mechanism of action by which an assay can be developed for bioefficacy evaluation of GZFLc.
American Psychological Association (APA)
Zheng, Weiran& Li, Meng& Wang, Yongxiang& Lv, Baojie& Zhang, Xinzhuang& Chen, Lilan…[et al.]. 2020. Guizhi Fuling Capsule Exhibits Antidysmenorrhea Activity by Inhibition of Cyclooxygenase Activity. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1157767
Modern Language Association (MLA)
Zheng, Weiran…[et al.]. Guizhi Fuling Capsule Exhibits Antidysmenorrhea Activity by Inhibition of Cyclooxygenase Activity. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-10.
https://search.emarefa.net/detail/BIM-1157767
American Medical Association (AMA)
Zheng, Weiran& Li, Meng& Wang, Yongxiang& Lv, Baojie& Zhang, Xinzhuang& Chen, Lilan…[et al.]. Guizhi Fuling Capsule Exhibits Antidysmenorrhea Activity by Inhibition of Cyclooxygenase Activity. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-10.
https://search.emarefa.net/detail/BIM-1157767
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157767