Genome-Wide Regulation of Electroacupuncture and Treadmill Exercise on Diet-Induced Obese Rats
Joint Authors
Wei, Dan
Mo, Guoyan
Zhang, Yanji
Li, Jia
Yu, Chaochao
Wang, Lihua
Zhuo, Yue
Xu, Kou
Zhang, Yingrong
Xue, Yixuan
Huang, Wei
Zhou, Zhongyu
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-25
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Acupuncture has been widely used for obesity treatment, but its mechanism is still unclear.
To investigate the molecular mechanisms, we applied electroacupuncture (EA) at the Zusanli (ST36) acupoint and treadmill exercise (TE) in a diet-induced obese (DIO) rat model and used RNA sequencing (RNA-seq) to identify molecular consequences.
Forty Sprague-Dawley male rats were selected and randomly divided into five groups: control (C), DIO model (M), EA, TE, and EA + TE groups.
According to the results, acupuncture reduced body weight and the ratio of retroperitoneal white adipose tissue (retro-WAT) to body weight.
Total RNA was extracted from the retro-WAT from five groups for RNA-seq.
Differentially expressed genes (DEG) analysis showed that there were obvious differences among the four comparisons of C vs.
M, M vs.
EA, M vs.
TE, and M vs.
EA + TE, followed by 1383, 913, 3324, and 2794 DE genes.
Gene ontology (GO) term enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis were performed to further classify the DEGs.
Several GO terms were commonly significantly enriched in both M vs.
TE and M vs.
EA, such as myofibril and muscle contraction.
In addition, some pathways were regulated by EA and TE, such as the peroxisome proliferator activated receptor signaling pathway and calcium signaling pathway.
This study is the first to compare and analyze the differences in gene expression profiles in the retro-WAT of rats in different groups, which provide a clue for further investigation into the molecular mechanisms of obesity treatment by EA and TE.
American Psychological Association (APA)
Zhang, Yanji& Li, Jia& Wei, Dan& Mo, Guoyan& Yu, Chaochao& Wang, Lihua…[et al.]. 2020. Genome-Wide Regulation of Electroacupuncture and Treadmill Exercise on Diet-Induced Obese Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1157947
Modern Language Association (MLA)
Zhang, Yanji…[et al.]. Genome-Wide Regulation of Electroacupuncture and Treadmill Exercise on Diet-Induced Obese Rats. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1157947
American Medical Association (AMA)
Zhang, Yanji& Li, Jia& Wei, Dan& Mo, Guoyan& Yu, Chaochao& Wang, Lihua…[et al.]. Genome-Wide Regulation of Electroacupuncture and Treadmill Exercise on Diet-Induced Obese Rats. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1157947
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1157947