In Vitro Antioxidant, Cytotoxic Activities, and Phenolic Profile of Senecio glaucus from Saudi Arabia
Joint Authors
Farooq, Muhammad
Alqahtani, Ali S.
Ullah, Riaz
Noman, Omar M.
Nasr, Fahd A
Herqash, Rashed N.
Alyhya, Nouf
Anazi, Shamsa H.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-10-24
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Current treatments for complex diseases have remarkable side effects that negatively impact patients’ quality of life.
Thus, natural compounds with fewer side effects represent a promising source for safe drugs.
The genus Senecio is widely used in folk medicine due to its various pharmacological properties.
In the present study, the total phenolic content of Senecio glaucus, which is grown in Saudi Arabia, was assessed using the Folin-Ciocalteau colorimetric method.
Scavenging DPPH and ABTS assays were utilized to determine the antioxidant properties of S.
glaucus fractions, and MTT assay was used to screen the cytotoxic activity of S.
glaucus against various cancer cells.
In addition, HPLC-UV was utilized to detect the presence of two phenolic acids, namely, vanillic acid (VA) and gallic acid (GA).
Among all fractions tested, S.
glaucus chloroform fraction (SGCF) yielded the highest value (125.3 mg·GA/g) in terms of total phenolic content.
SGCF also exhibited the highest scavenging activities (76.7 and 74.1%) on both DPPH and ABTS assays, respectively.
Similarly, SGCF also possessed the most potent cytotoxic activity against the MCF-7 cell line, with an IC50 value of 41.8 μg/ml.
The validated HPLC method confirmed the presence of VA (4.8 μg/mg DW) and GA (3.9 μg/mg DW) in SGCF.
Overall, our data show that S.
glaucus had antioxidant and cytotoxic properties.
A developed validated HPLC method which could be helpful for quantifying phenolic compounds in S.
glaucus was established.
American Psychological Association (APA)
Alqahtani, Ali S.& Herqash, Rashed N.& Noman, Omar M.& Nasr, Fahd A& Alyhya, Nouf& Anazi, Shamsa H.…[et al.]. 2020. In Vitro Antioxidant, Cytotoxic Activities, and Phenolic Profile of Senecio glaucus from Saudi Arabia. Evidence-Based Complementary and Alternative Medicine،Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1158162
Modern Language Association (MLA)
Alqahtani, Ali S.…[et al.]. In Vitro Antioxidant, Cytotoxic Activities, and Phenolic Profile of Senecio glaucus from Saudi Arabia. Evidence-Based Complementary and Alternative Medicine No. 2020 (2020), pp.1-9.
https://search.emarefa.net/detail/BIM-1158162
American Medical Association (AMA)
Alqahtani, Ali S.& Herqash, Rashed N.& Noman, Omar M.& Nasr, Fahd A& Alyhya, Nouf& Anazi, Shamsa H.…[et al.]. In Vitro Antioxidant, Cytotoxic Activities, and Phenolic Profile of Senecio glaucus from Saudi Arabia. Evidence-Based Complementary and Alternative Medicine. 2020. Vol. 2020, no. 2020, pp.1-9.
https://search.emarefa.net/detail/BIM-1158162
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1158162