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miR-129-5p Inhibits Adipogenesis through Autophagy and May Be a Potential Biomarker for Obesity
Joint Authors
Mu, Qian
Ning, Guang
Fu, Xue
Jin, Lina
Han, Luyu
Yuan, Yini
Wang, Hui
Yang, Jian
Zhou, Donglei
Zhang, Zhiguo
Source
International Journal of Endocrinology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-11-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Introduction.
Obesity has an unclear pathogenesis.
MicroRNAs (miRNAs) may function as biologically active molecules for obesity through regulating adipocyte differentiation.
This study aimed to identify how miR-129-5p (a specific miRNA) regulates adipogenesis in vitro and explore its possible role in the pathogenesis of obesity in humans.
Materials and Methods.
The miR-129-5p expression was detected in obese mouse models.
The effect of miR-129-5p on adipocyte differentiation was observed, and the adipose markers were analyzed.
Bioinformatics and dual-luciferase reporter assay were applied to predict and confirm the target genes of miR-129-5p.
The human serum samples were detected and analyzed.
Results.
miR-129-5p is highly expressed in adipose tissues of db/db mice.
Gain- and loss-of-function studies show that miR-129-5p could significantly inhibit adipocyte differentiation and white adipocyte browning in vitro and decreases the level of specific markers, such as FABP4, UCP1, and PPARγ, in mature white and brown adipocytes.
miR-129-5p directly targets ATG7 which is predicted with bioinformatics and confirmed by dual-luciferase reporter assay.
Serum miR-129-5p level was evidently elevated in patients with simple obesity (p<0.01) and correlates with obesity indices, including BMI (r = 0.407, p<0.029) and fat percentage (r = 0.394, p<0.038).
Conclusion.
miR-129-5p might target on the ATG7-related autophagy signaling network that regulates white and brown adipogenesis.
Importantly, the aforementioned results suggest serum miR-129-5p might be a potential biomarker and therapeutic target for obesity.
American Psychological Association (APA)
Fu, Xue& Jin, Lina& Han, Luyu& Yuan, Yini& Mu, Qian& Wang, Hui…[et al.]. 2019. miR-129-5p Inhibits Adipogenesis through Autophagy and May Be a Potential Biomarker for Obesity. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1159582
Modern Language Association (MLA)
Fu, Xue…[et al.]. miR-129-5p Inhibits Adipogenesis through Autophagy and May Be a Potential Biomarker for Obesity. International Journal of Endocrinology No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1159582
American Medical Association (AMA)
Fu, Xue& Jin, Lina& Han, Luyu& Yuan, Yini& Mu, Qian& Wang, Hui…[et al.]. miR-129-5p Inhibits Adipogenesis through Autophagy and May Be a Potential Biomarker for Obesity. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1159582
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1159582