Effects of Exposure to Bisphenol A during Pregnancy on the Pup Testis Function
Joint Authors
Yang, Qingtao
Sui, Xuxia
Cao, Junjun
Liu, Caixia
Zheng, Shukai
Bao, Mian
Huang, Yuanni
Wu, Kusheng
Source
International Journal of Endocrinology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-21
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Testosterone plays an important prenatal role in male testis development.
Bisphenol A (BPA) exposure during pregnancy affects testosterone levels and germ cell apoptosis of male pups, but little information is available for the mechanism.
The aim of the present study was to investigate the mechanism by which BPA alters testosterone levels and germ cell apoptosis.
Pregnant female C57BL/6J mice, throughout gestation, had access to drinking water containing BPA at 5 and 50 μg/mL.
Male pups were euthanized on postnatal days (PNDs) 1, 14, and 35.
Relative to control, BPA exposure at 5 and 50 μg/ml decreased testosterone level, as measured by chemiluminescent immunoassay, on PND14.
Real-time PCR indicated mRNA levels for steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (CYP11A1), and 3-β-hydroxysteroid dehydrogenase/△-5-4 isomerase (3β-HSD) were significantly lower in the BPA pups compared to control.
Additionally, BPA increased the percentage of TUNEL-positive seminiferous tubules, decreased the mRNA level of Bcl-2, and increased Bax expression, indicative of increased apoptosis.
These results suggest that BPA exposure in utero decreases the testosterone concentration by decreasing steroidogenic enzymes (StAR, CYP11A1, and 3β-HSD).
Furthermore, BPA exposure increases the apoptosis of germ cells, which is associated with proapoptotic changes in the levels of Bcl-2 and Bax.
American Psychological Association (APA)
Yang, Qingtao& Sui, Xuxia& Cao, Junjun& Liu, Caixia& Zheng, Shukai& Bao, Mian…[et al.]. 2019. Effects of Exposure to Bisphenol A during Pregnancy on the Pup Testis Function. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1159658
Modern Language Association (MLA)
Yang, Qingtao…[et al.]. Effects of Exposure to Bisphenol A during Pregnancy on the Pup Testis Function. International Journal of Endocrinology No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-1159658
American Medical Association (AMA)
Yang, Qingtao& Sui, Xuxia& Cao, Junjun& Liu, Caixia& Zheng, Shukai& Bao, Mian…[et al.]. Effects of Exposure to Bisphenol A during Pregnancy on the Pup Testis Function. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-1159658
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1159658