Comparative Proteome Analysis of Epicardial and Subcutaneous Adipose Tissues from Patients with or without Coronary Artery Disease
Joint Authors
Gong, Fengying
Zhu, Hui juan
Pan, Hui
Li, Naishi
Wang, Lin jie
Yang, Hong bo
Sun, Wei
Zhao, Yu xing
Liu, Xue mei
Zeng, Yong
Source
International Journal of Endocrinology
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-08-25
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background and Aims.
Owing to its unique anatomical structure and metabolism, epicardial adipose tissue (EAT) has attracted amount of attention in coronary artery disease (CAD) research.
Here, we analyzed differences in proteome composition in epicardial (EAT) and subcutaneous adipose tissues (SAT) from patients with or without CAD.
Methods.
EAT and SAT samples were collected from 6 CAD patients and 6 non-CAD patients.
Isobaric Tagging for Relative and Absolute Quantitation (iTRAQ) analysis combined with liquid chromatography tandem-mass spectrometry (LC-MS/MS) was performed to identify the differentially expressed proteins.
Results.
In total, 2348 proteins expressed in EAT and 2347 proteins expressed in SAT were separately identified.
385 differentially expressed proteins were found in EAT and 210 proteins were found in SAT in CAD patients compared to non-CAD patients.
Many proteins differentially expressed in EAT of CAD patients were involved in biological functions associated with CAD development such as cell-to-cell signaling and interaction, inflammatory response, and lipid metabolism.
Differential expressions of proteins (MMP9, S100A9, and clusterin) in EAT or SAT were involved in several signaling pathways such as mitochondrial dysfunction, acute phase inflammation, and LXR/RXR activation, which was confirmed by western blotting, and similar results were obtained.
Conclusions.
The largest profiles of differentially expressed proteins in EAT and SAT between CAD patients and non-CAD patients were identified.
The significant signal pathways, mitochondrial dysfunction, and LXR/RXR activation, which differential proteins were involved in, were firstly found to play roles in EAT of CAD patients, and clusterin was firstly found to be upregulated in EAT of CAD patients.
American Psychological Association (APA)
Zhao, Yu xing& Zhu, Hui juan& Pan, Hui& Liu, Xue mei& Wang, Lin jie& Yang, Hong bo…[et al.]. 2019. Comparative Proteome Analysis of Epicardial and Subcutaneous Adipose Tissues from Patients with or without Coronary Artery Disease. International Journal of Endocrinology،Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1159672
Modern Language Association (MLA)
Zhao, Yu xing…[et al.]. Comparative Proteome Analysis of Epicardial and Subcutaneous Adipose Tissues from Patients with or without Coronary Artery Disease. International Journal of Endocrinology No. 2019 (2019), pp.1-11.
https://search.emarefa.net/detail/BIM-1159672
American Medical Association (AMA)
Zhao, Yu xing& Zhu, Hui juan& Pan, Hui& Liu, Xue mei& Wang, Lin jie& Yang, Hong bo…[et al.]. Comparative Proteome Analysis of Epicardial and Subcutaneous Adipose Tissues from Patients with or without Coronary Artery Disease. International Journal of Endocrinology. 2019. Vol. 2019, no. 2019, pp.1-11.
https://search.emarefa.net/detail/BIM-1159672
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1159672