Enriching Genomic Resources and Transcriptional Profile Analysis of Miscanthus sinensis under Drought Stress Based on RNA Sequencing
Joint Authors
Nie, Gang
Huang, Linkai
Ma, Xiao
Ji, Zhongjie
Zhang, Yajie
Tang, Lu
Zhang, Xinquan
Source
International Journal of Genomics
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-29
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Miscanthus × giganteus is wildly cultivated as a potential biofuel feedstock around the world; however, the narrow genetic basis and sterile characteristics have become a limitation for its utilization.
As a progenitor of M. × giganteus, M.
sinensis is widely distributed around East Asia providing well abiotic stress tolerance.
To enrich the M.
sinensis genomic databases and resources, we sequenced and annotated the transcriptome of M.
sinensis by using an Illumina HiSeq 2000 platform.
Approximately 316 million high-quality trimmed reads were generated from 349 million raw reads, and a total of 114,747 unigenes were obtained after de novo assembly.
Furthermore, 95,897 (83.57%) unigenes were annotated to at least one database including NR, Swiss-Prot, KEGG, COG, GO, and NT, supporting that the sequences obtained were annotated properly.
Differentially expressed gene analysis indicates that drought stress 15 days could be a critical period for M.
sinensis response to drought stress.
The high-throughput transcriptome sequencing of M.
sinensis under drought stress has greatly enriched the current genomic available resources.
The comparison of DEGs under different periods of drought stress identified a wealth of candidate genes involved in drought tolerance regulatory networks, which will facilitate further genetic improvement and molecular studies of the M.
sinensis.
American Psychological Association (APA)
Nie, Gang& Huang, Linkai& Ma, Xiao& Ji, Zhongjie& Zhang, Yajie& Tang, Lu…[et al.]. 2017. Enriching Genomic Resources and Transcriptional Profile Analysis of Miscanthus sinensis under Drought Stress Based on RNA Sequencing. International Journal of Genomics،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1167267
Modern Language Association (MLA)
Nie, Gang…[et al.]. Enriching Genomic Resources and Transcriptional Profile Analysis of Miscanthus sinensis under Drought Stress Based on RNA Sequencing. International Journal of Genomics No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1167267
American Medical Association (AMA)
Nie, Gang& Huang, Linkai& Ma, Xiao& Ji, Zhongjie& Zhang, Yajie& Tang, Lu…[et al.]. Enriching Genomic Resources and Transcriptional Profile Analysis of Miscanthus sinensis under Drought Stress Based on RNA Sequencing. International Journal of Genomics. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1167267
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167267