Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model
Joint Authors
Spies, Claudia D.
von Haefen, Clarissa
Wernecke, Klaus-Dieter
Lanzke, Nadine
Menk, Mario
Sargsyan, Lilit
Scharf, Bianca
Source
International Journal of Inflammation
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-11-20
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Background.
Interactions between alcohol, infection, and surgery and their effect on differentiation and functionality of T helper cells are not yet completely understood.
We hypothesized that alcohol and surgery disturb differentiation of T helper cells and contribute to an impaired immune response.
Methods.
Mice were treated with alcohol for two weeks.
Saline treatment served as control.
Clinical performance and weight were assessed.
On day 14, a median laparotomy was performed and animals were challenged with Klebsiella pneumoniae intranasally.
Bacterial load was determined in lungs and blood.
T helper cell subpopulations and the released cytokines were assessed in lungs, spleens, and plasma.
Key transcription factors of T cell differentiation were evaluated.
Results.
Alcohol significantly impaired clinical appearance and body weight of animals with postsurgical infection (p<0.05).
Bacterial load was significantly higher after alcohol treatment (p<0.05).
T helper cell subsets and released cytokine levels were significantly altered in lung, but not in spleen.
Expression of transcription factors of T helper cell lineage commitment did not translate into different counts of T helper cells.
Conclusions.
Alcohol and surgery lead to significant cellular and functional modulations of T helper cells during postsurgical infection.
These effects may contribute to an impaired immune response after surgery.
American Psychological Association (APA)
Lanzke, Nadine& Menk, Mario& von Haefen, Clarissa& Sargsyan, Lilit& Scharf, Bianca& Wernecke, Klaus-Dieter…[et al.]. 2017. Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model. International Journal of Inflammation،Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1167420
Modern Language Association (MLA)
Lanzke, Nadine…[et al.]. Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model. International Journal of Inflammation No. 2017 (2017), pp.1-14.
https://search.emarefa.net/detail/BIM-1167420
American Medical Association (AMA)
Lanzke, Nadine& Menk, Mario& von Haefen, Clarissa& Sargsyan, Lilit& Scharf, Bianca& Wernecke, Klaus-Dieter…[et al.]. Ethanol-Induced Alterations of T Cells and Cytokines after Surgery in a Murine Infection Model. International Journal of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-14.
https://search.emarefa.net/detail/BIM-1167420
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167420