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Transcriptional and Molecular Pathways Activated in Mesenteric Adipose Tissue and Intestinal Mucosa of Crohn’s Disease Patients
Joint Authors
Milanski, Marciane
Ayrizono, Maria de Lourdes Setsuko
Camargo, Michel Gardere
Silva, Francesca A. R.
Leal, Raquel F.
Coope, Andressa
Pascoal, Lívia Bitencourt
Botezelli, José Diego
Planell, Núria
Portovedo, Mariana
Dias, Cilene Bicca
Fagundes, João José
Source
International Journal of Inflammation
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-04-09
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Crohn’s disease (CD) is a chronic inflammatory disorder, characterized by cytokine imbalance and transcription signaling pathways activation.
In addition, the increase of mesenteric adipose tissue (MAT) near the affected intestinal area is a hallmark of CD.
Therefore, we evaluated the transcription signaling pathways and cytokines expression in intestinal mucosa and MAT of active CD patients.
Ten patients with ileocecal CD and eight with noninflammatory diseases were studied.
The biopsies of intestinal mucosa and MAT were snap-frozen and protein expression was determined by immunoblotting.
RNA levels were measured by qPCR.
The pIkB/IkB ratio and TNFα level were significantly higher in intestinal mucosa of CD when compared to controls.
However, STAT1 expression was similar between intestinal mucosa of CD and controls.
Considering the MAT, the pIkB/IkB ratio was significantly lower and the anti-inflammatory cytokine IL10 was significantly higher in CD when compared to controls.
Finally, the protein content of pSTAT1 was higher in MAT of CD compared to controls.
These findings reinforce the predominance of the proinflammatory NF-kB pathway in CD intestinal mucosa.
For the first time, we showed the activation of STAT1 pathway in MAT of CD patients, which may help to understand the physiopathology of this immune mediated disease.
American Psychological Association (APA)
Coope, Andressa& Pascoal, Lívia Bitencourt& Silva, Francesca A. R.& Botezelli, José Diego& Ayrizono, Maria de Lourdes Setsuko& Milanski, Marciane…[et al.]. 2017. Transcriptional and Molecular Pathways Activated in Mesenteric Adipose Tissue and Intestinal Mucosa of Crohn’s Disease Patients. International Journal of Inflammation،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1167468
Modern Language Association (MLA)
Coope, Andressa…[et al.]. Transcriptional and Molecular Pathways Activated in Mesenteric Adipose Tissue and Intestinal Mucosa of Crohn’s Disease Patients. International Journal of Inflammation No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1167468
American Medical Association (AMA)
Coope, Andressa& Pascoal, Lívia Bitencourt& Silva, Francesca A. R.& Botezelli, José Diego& Ayrizono, Maria de Lourdes Setsuko& Milanski, Marciane…[et al.]. Transcriptional and Molecular Pathways Activated in Mesenteric Adipose Tissue and Intestinal Mucosa of Crohn’s Disease Patients. International Journal of Inflammation. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1167468
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167468