Development and Validation of UPLC-MSMS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application
Joint Authors
Qiu, Xiang-jun
Li, Shuang-long
Zhu, Yong-liang
Zhang, Yi
Liu, Shu-han
Wang, Xiang-die
Source
International Journal of Analytical Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-03-31
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
In our research, a straightforward UPLC-MS/MS method, with diazepam as the internal standard (IS), was proposed and acknowledged to determine the concentrations of enasidenib in rat plasma.
When preparing the sample, we used acetonitrile for protein precipitation.
The gradient elution method was used, and the mobile phase was acetonitrile and 0.1% formic acid.
Diazepam was used as the IS.
We used the Acquity UPLC BEH C18 column to separate enasidenib and IS.
Under the positive ion electrospray ionization (ESI) source conditions, the mass transfer pairs of enasidenib were monitored by multiple reaction monitoring (MRM) to be m/z 474.2 ⟶ 456.1 and m/z 474.2 ⟶ 267.0, and the IS mass transfer pairs were m/z 285.0 ⟶ 154.0.
Enasidenib had good linearity (r2 = 0.9985) in the concentration range of 1.0–1000 ng/mL.
Besides, the values of intraday and interday precision were 2.25–8.40% and 3.94–5.46%, respectively, and the range of the accuracy values varied from −1.44 to 2.34%.
Matrix effect, extraction recovery, and stability were compliant with FDA approval guidelines in terms of bioanalytical method validation.
We had established a new method that had been applied to the pharmacokinetic study of enasidenib in rats.
American Psychological Association (APA)
Li, Shuang-long& Zhu, Yong-liang& Zhang, Yi& Liu, Shu-han& Wang, Xiang-die& Qiu, Xiang-jun. 2020. Development and Validation of UPLC-MSMS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167658
Modern Language Association (MLA)
Li, Shuang-long…[et al.]. Development and Validation of UPLC-MSMS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1167658
American Medical Association (AMA)
Li, Shuang-long& Zhu, Yong-liang& Zhang, Yi& Liu, Shu-han& Wang, Xiang-die& Qiu, Xiang-jun. Development and Validation of UPLC-MSMS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167658
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167658