Development and Validation of UPLC-MSMS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application

Joint Authors

Qiu, Xiang-jun
Li, Shuang-long
Zhu, Yong-liang
Zhang, Yi
Liu, Shu-han
Wang, Xiang-die

Source

International Journal of Analytical Chemistry

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-03-31

Country of Publication

Egypt

No. of Pages

7

Main Subjects

Chemistry
Science

Abstract EN

In our research, a straightforward UPLC-MS/MS method, with diazepam as the internal standard (IS), was proposed and acknowledged to determine the concentrations of enasidenib in rat plasma.

When preparing the sample, we used acetonitrile for protein precipitation.

The gradient elution method was used, and the mobile phase was acetonitrile and 0.1% formic acid.

Diazepam was used as the IS.

We used the Acquity UPLC BEH C18 column to separate enasidenib and IS.

Under the positive ion electrospray ionization (ESI) source conditions, the mass transfer pairs of enasidenib were monitored by multiple reaction monitoring (MRM) to be m/z 474.2 ⟶ 456.1 and m/z 474.2 ⟶ 267.0, and the IS mass transfer pairs were m/z 285.0 ⟶ 154.0.

Enasidenib had good linearity (r2 = 0.9985) in the concentration range of 1.0–1000 ng/mL.

Besides, the values of intraday and interday precision were 2.25–8.40% and 3.94–5.46%, respectively, and the range of the accuracy values varied from −1.44 to 2.34%.

Matrix effect, extraction recovery, and stability were compliant with FDA approval guidelines in terms of bioanalytical method validation.

We had established a new method that had been applied to the pharmacokinetic study of enasidenib in rats.

American Psychological Association (APA)

Li, Shuang-long& Zhu, Yong-liang& Zhang, Yi& Liu, Shu-han& Wang, Xiang-die& Qiu, Xiang-jun. 2020. Development and Validation of UPLC-MSMS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167658

Modern Language Association (MLA)

Qiu, Xiang-jun…[et al.]. Development and Validation of UPLC-MSMS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1167658

American Medical Association (AMA)

Li, Shuang-long& Zhu, Yong-liang& Zhang, Yi& Liu, Shu-han& Wang, Xiang-die& Qiu, Xiang-jun. Development and Validation of UPLC-MSMS Method for Determination of Enasidenib in Rat Plasma and Its Pharmacokinetic Application. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167658

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1167658