Extraction-Free, Direct Determination of Caffeine in Microliter Volumes of Beverages by Thermal Desorption-Gas Chromatography Mass Spectrometry
Joint Authors
Honeychurch, Kevin
Peng, Xianglu
Brown, Melanie
Bowdler, Paul
Source
International Journal of Analytical Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-04-01
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
An extraction-free method requiring microliter (μL) volumes has been developed for the determination of caffeine in beverages.
Using a pyrolysis-gas chromatography mass spectrometry system, the conditions required for the direct thermal desorption-gas chromatography mass spectrometry (TD-GC/MS) determination of caffeine were optimised.
A 5 μL aliquot was introduced to the thermal desorption unit, dried, and thermally desorbed to the GC/MS.
The response was linear over the range 10 to 500 μg/mL (R2 = 0.996).
The theoretical limit of detection (3 σ) was 0.456 μg/mL.
No interferences were recorded from endogenous beverage components or from commonly occurring drugs, such as nicotine, ibuprofen, and paracetamol.
Replicate caffeine determinations on fortified latte style white coffee and Pepsi Max® gave mean recoveries of 93.4% (%CV = 4.1%) and 95.0% (%CV = 0.98%), respectively.
Good agreement was also obtained with the stated values of caffeine for an energy drink and for Coca-Cola®.
These data suggest that the method holds promise for the determination of caffeine in such samples.
American Psychological Association (APA)
Peng, Xianglu& Brown, Melanie& Bowdler, Paul& Honeychurch, Kevin. 2020. Extraction-Free, Direct Determination of Caffeine in Microliter Volumes of Beverages by Thermal Desorption-Gas Chromatography Mass Spectrometry. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167660
Modern Language Association (MLA)
Peng, Xianglu…[et al.]. Extraction-Free, Direct Determination of Caffeine in Microliter Volumes of Beverages by Thermal Desorption-Gas Chromatography Mass Spectrometry. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1167660
American Medical Association (AMA)
Peng, Xianglu& Brown, Melanie& Bowdler, Paul& Honeychurch, Kevin. Extraction-Free, Direct Determination of Caffeine in Microliter Volumes of Beverages by Thermal Desorption-Gas Chromatography Mass Spectrometry. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167660
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167660