Systematic Analysis of the Whole-Body Tissue Distribution and Fatty Acid Compositions of Membrane Lipids in CD1 and NMRI Mice and Wistar Rats
Joint Authors
Xu, Lina
Reichel, Andreas
Wang, Xueying
Schmitt, Maximilian V.
Ruan, Huabin
Jiao, Yupei
Wang, Yusong
Lienau, Philip
Liu, Xiaohui
Yang, Tao
Source
International Journal of Analytical Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-11-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Understanding the tissue distribution of phospholipids and glycerolipids in animal models enables promoting the pharmacokinetic study of drugs and related PK predictions.
The measurement of lipid compositions in animal models, usually mice and rats, without a standardized approach hindered the accuracy of PBPK investigation.
In this work, high resolution mass spectrometry was applied to profile the tissue distribution of phospholipids and glycerolipids in 12 organs/tissues of mice and rats.
Using this method, not only the amounts of phospholipids and glycerolipids in each organ/tissue but also the fatty acid compositions were acquired.
In order to explore the interspecies specificity of lipid distribution in different organs/tissues, three animal species including CD1 mice, NMRI mice, and Wister rats were used in this systematic study.
Globally, more organ specificity was observed.
It was found that the brain is the organ containing the most abundant phosphatidylserine lipids (PSs) in all three animal models, leading to brain tissues having the most concentrated acidic phospholipids.
Diverse fatty acid compositions in each lipid class were clearly revealed.
Certain tissues/organs also had a specific selection of unique fatty acid compositions, for example, unreferenced FA(18 : 2) in the brain.
It turned out that the access of free fatty acids affects the incorporation of acyl chain in phospholipids and glycerolipids.
In the analysis, ether lipids were also profiled with the observation of dominant ePEs in brain tissues.
However, little interspecies difference was found for fatty acid constituents and tissues distribution of phospholipids and glycerolipids.
American Psychological Association (APA)
Xu, Lina& Schmitt, Maximilian V.& Ruan, Huabin& Jiao, Yupei& Wang, Xueying& Wang, Yusong…[et al.]. 2020. Systematic Analysis of the Whole-Body Tissue Distribution and Fatty Acid Compositions of Membrane Lipids in CD1 and NMRI Mice and Wistar Rats. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1167738
Modern Language Association (MLA)
Xu, Lina…[et al.]. Systematic Analysis of the Whole-Body Tissue Distribution and Fatty Acid Compositions of Membrane Lipids in CD1 and NMRI Mice and Wistar Rats. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1167738
American Medical Association (AMA)
Xu, Lina& Schmitt, Maximilian V.& Ruan, Huabin& Jiao, Yupei& Wang, Xueying& Wang, Yusong…[et al.]. Systematic Analysis of the Whole-Body Tissue Distribution and Fatty Acid Compositions of Membrane Lipids in CD1 and NMRI Mice and Wistar Rats. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1167738
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167738