A Validated Liquid Chromatographic Method for Berberine Analysis in Tissue and Application
Joint Authors
Zhou, Neng
Liu, Dangmei
Bao, Xiaowang
Source
International Journal of Analytical Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Simple and rapid high-performance liquid chromatography methods were developed for the determination of berberine (BB) in various rat tissues so as to evaluate a P-gp inhibitor, glycyrrhetinic acid (GA), on BB’s oral bioavailability.
Acetonitrile was used to extract BB from tissues and showed different extraction recoveries in diverse tissues.
The intra- and interday precision and accuracy were less than 10%.
Long-term stability, pre (post) -preparative stability, and freeze-thaw stability were evaluated, and the results showed it could meet the need of this study.
The proposed methods were subsequently applied to investigate the possible drug-drug interaction of GA and BB in vivo from the aspect of tissue distribution.
The results showed that no significant difference was found between the group of low dose and high dose at the same time point.
The tissue distributions show a saturated model, i.e., the content of BB in tissue tends to be constant while its dose is more than 200 mg/kg.
Besides, the contents of BB ranged from high to low according to the order of the liver, kidney, and spleen.
The BB content in the liver is especially high as compared to other tissues.
American Psychological Association (APA)
Zhou, Neng& Liu, Dangmei& Bao, Xiaowang. 2020. A Validated Liquid Chromatographic Method for Berberine Analysis in Tissue and Application. International Journal of Analytical Chemistry،Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167807
Modern Language Association (MLA)
Zhou, Neng…[et al.]. A Validated Liquid Chromatographic Method for Berberine Analysis in Tissue and Application. International Journal of Analytical Chemistry No. 2020 (2020), pp.1-7.
https://search.emarefa.net/detail/BIM-1167807
American Medical Association (AMA)
Zhou, Neng& Liu, Dangmei& Bao, Xiaowang. A Validated Liquid Chromatographic Method for Berberine Analysis in Tissue and Application. International Journal of Analytical Chemistry. 2020. Vol. 2020, no. 2020, pp.1-7.
https://search.emarefa.net/detail/BIM-1167807
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1167807