Development of a Sensitive Chemiluminescence Immunoassay for the Quantification of Folic Acid in Human Serum
Joint Authors
Zhang, Ting
Chen, Xiang
Zhou, Qiyang
Wang, ChunXin
Yu, Zheng
Ahamada, Hadji
Bai, Zhonghu
Huang, Xuan
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-05-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Folic acid (FA) is an important vitamin for human growth, especially for pregnant women.
FA deficiency is associated with megaloblastic anemia, neural tube defects, cardiovascular diseases, irritability, diarrhea, and psychiatric disorders.
Normally, FA molecules bind to folate-binding protein (FBP) in the serum as complex.
Before quantify the FA concentration, a releasing procedure should be conducted.
Alkaline condition and tris(2-carboxyethyl)phosphine (TCEP) are used to release binding FA to freeing state.
In this work, a chemiluminescence immunoassay (CLIA) for human serum FA was established by competition model.
Streptavidin (SA) was labeled to magnetic beads by an 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDAC/NHS) method.
Activated biotin molecules were labeled to FBP molecules purified from milk.
FA was labeled to horseradish peroxidase (HRP) by EDAC to activate the FA molecules.
The pretreated samples or standards were added into the reaction tube with biotin-FBP and FA-horseradish peroxidase (HRP), FA in the sample compete with FA-HRP for binding to biotin-FBP, the signal is inversely proportional to the FA concentration.
The method established shows good thermostability and performance.
The limitation of detection (LOD) is 0.44 ng/mL.
The intra-assay coefficient of variation (CV) is 3.6%–7.1%, the interassay CV is 4.2%–7.5%, and the recovery rate is 92.1%–103.5%.
Cross reactivity (CR) was remarkably low with aminopterin, folinic acid, and methotrexate.
The method shows good correlation with the FA CLIA product from Beckman Coulter; the equation is y = 0.9618x−0.1434 while the R2 value is 0.9224.
The established method is sensitive, rapid, and accurate which can fully satisfy for the clinical requirement.
American Psychological Association (APA)
Chen, Xiang& Zhou, Qiyang& Zhang, Ting& Wang, ChunXin& Yu, Zheng& Ahamada, Hadji…[et al.]. 2019. Development of a Sensitive Chemiluminescence Immunoassay for the Quantification of Folic Acid in Human Serum. Journal of Analytical Methods in Chemistry،Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1169194
Modern Language Association (MLA)
Chen, Xiang…[et al.]. Development of a Sensitive Chemiluminescence Immunoassay for the Quantification of Folic Acid in Human Serum. Journal of Analytical Methods in Chemistry No. 2019 (2019), pp.1-7.
https://search.emarefa.net/detail/BIM-1169194
American Medical Association (AMA)
Chen, Xiang& Zhou, Qiyang& Zhang, Ting& Wang, ChunXin& Yu, Zheng& Ahamada, Hadji…[et al.]. Development of a Sensitive Chemiluminescence Immunoassay for the Quantification of Folic Acid in Human Serum. Journal of Analytical Methods in Chemistry. 2019. Vol. 2019, no. 2019, pp.1-7.
https://search.emarefa.net/detail/BIM-1169194
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1169194