Lactobacillus rhamnosus Reduces Blood Glucose Level through Downregulation of Gluconeogenesis Gene Expression in Streptozotocin-Induced Diabetic Rats
Joint Authors
Farida, Eko
Nuraida, Lilis
Giriwono, Puspo E.
Jenie, Betty S. L.
Source
International Journal of Food Science
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-01-13
Country of Publication
Egypt
No. of Pages
12
Abstract EN
Some lactic acid bacteria (LAB) are observed to be potential probiotics with functional properties such as lowering fasting blood glucose (FBG), as a promising hyperglycemia management.
This study investigated the ability and mechanism of Lactobacillus rhamnosus BSL and Lactobacillus rhamnosus R23 on lowering FBG in diabetic rats induced by streptozotocin (STZ).
The rats were orally administered with L.
rhamnosus BSL and L.
rhamnosus R23 by giving 1 mL cell suspension (109 CFU/mL) daily for 30 days.
The body weight (BW) was recorded once in three days, and FBG was recorded once in six days.
An oral glucose tolerance test (OGTT) was measured 1 week after injection with STZ and before sacrifice.
Fecal samples were collected on days 0, 15, and 30 for LAB population and identification, performed by PCR detecting 16S rRNA.
Oral administration of L.
rhamnosus BSL and L.
rhamnosus R23 decreased FBG and improved glucose tolerance via downregulation of glucose-6-phosphatase (G6pc) expression by 0.57- and 0.60-fold change, respectively (P<0.05).
The lipid profiles, BUN, creatinine, SGOT, and SGPT were significantly (P<0.05) different between normal and diabetic rats, but they were not significantly (P>0.05) different among diabetic rats.
Both strains were effective in increasing fecal LAB population.
Molecular identification of the isolated LAB from fecal sample indicated that they were able to survive and pass through the digestive tract.
These results suggested that both strains have the ability to manage blood glucose level and become a promising agent to manage hyperglycemia and diabetes.
American Psychological Association (APA)
Farida, Eko& Nuraida, Lilis& Giriwono, Puspo E.& Jenie, Betty S. L.. 2020. Lactobacillus rhamnosus Reduces Blood Glucose Level through Downregulation of Gluconeogenesis Gene Expression in Streptozotocin-Induced Diabetic Rats. International Journal of Food Science،Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1171019
Modern Language Association (MLA)
Farida, Eko…[et al.]. Lactobacillus rhamnosus Reduces Blood Glucose Level through Downregulation of Gluconeogenesis Gene Expression in Streptozotocin-Induced Diabetic Rats. International Journal of Food Science No. 2020 (2020), pp.1-12.
https://search.emarefa.net/detail/BIM-1171019
American Medical Association (AMA)
Farida, Eko& Nuraida, Lilis& Giriwono, Puspo E.& Jenie, Betty S. L.. Lactobacillus rhamnosus Reduces Blood Glucose Level through Downregulation of Gluconeogenesis Gene Expression in Streptozotocin-Induced Diabetic Rats. International Journal of Food Science. 2020. Vol. 2020, no. 2020, pp.1-12.
https://search.emarefa.net/detail/BIM-1171019
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171019