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Effects of Cholecalciferol on Key Components of Vitamin D-EndoParaAutocrine System in Experimental Type 1 Diabetes
Joint Authors
Mazanova, Anna
Shymanskyi, Ihor
Lisakovska, Olha
Hajiyeva, Lala
Komisarenko, Yulia
Veliky, Mykola
Source
International Journal of Endocrinology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-02-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Objectives.
Recent prospective studies have found the associations between type 1 diabetes (T1D) and vitamin D deficiency.
We investigated the role of vitamin D in the regulation of 25OHD-1α-hydroxylase (CYP27B1) and VDR expression in different tissues of T1D rats.
Design.
T1D was induced in male Wistar rats by streptozotocin (55 mg/k b.w.).
After 2 weeks of T1D, the animals were treated orally with or without vitamin D3 (cholecalciferol; 100 IU/rat, 30 days).
Methods.
Serum 25-hydroxyvitamin D (25OHD) was detected by ELISA.
CYP27A1, CYP2R1, CYP27B1, and VDR were assayed by RT-qPCR and Western blotting or visualized by immunofluorescence staining.
Results.
We demonstrated that T1D led to a decrease in blood 25OHD, which is probably due to the established downregulation of CYP27A1 and CYP2R1 expression.
Vitamin D deficiency was accompanied by elevated synthesis of renal CYP27B1 and VDR.
Conversely, CYP27B1 and VDR expression decreased in the liver, bone tissue, and bone marrow.
Cholecalciferol administration countered the impairments of the vitamin D-endo/para/autocrine system in the kidneys and extrarenal tissues of diabetic rats.
Conclusions.
T1D-induced vitamin D deficiency is associated with impairments of renal and extrarenal CYP27B1 and VDR expression.
Cholecalciferol can be effective in the amelioration of diabetes-associated abnormalities in the vitamin D-endo/para/autocrine system.
American Psychological Association (APA)
Mazanova, Anna& Shymanskyi, Ihor& Lisakovska, Olha& Hajiyeva, Lala& Komisarenko, Yulia& Veliky, Mykola. 2018. Effects of Cholecalciferol on Key Components of Vitamin D-EndoParaAutocrine System in Experimental Type 1 Diabetes. International Journal of Endocrinology،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1171174
Modern Language Association (MLA)
Mazanova, Anna…[et al.]. Effects of Cholecalciferol on Key Components of Vitamin D-EndoParaAutocrine System in Experimental Type 1 Diabetes. International Journal of Endocrinology No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1171174
American Medical Association (AMA)
Mazanova, Anna& Shymanskyi, Ihor& Lisakovska, Olha& Hajiyeva, Lala& Komisarenko, Yulia& Veliky, Mykola. Effects of Cholecalciferol on Key Components of Vitamin D-EndoParaAutocrine System in Experimental Type 1 Diabetes. International Journal of Endocrinology. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1171174
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171174