The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001
Joint Authors
Tian, Kejian
Huo, Hongliang
Meng, Fanxing
Meng, Qi
Gao, Yan
Zhang, Lili
Wang, Le
Wang, Yuqing
Li, Xue
Source
International Journal of Genomics
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-08-26
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Estrogen contamination is recognized as one of the most serious environmental problems, causing widespread concern worldwide.
Environmental estrogens are mainly derived from human and vertebrate excretion, drugs, and agricultural activities.
The use of microorganisms is currently the most economical and effective method for biodegradation of environmental estrogens.
Rhodococcus equi DSSKP-R-001 (R-001) has strong estrogen-degrading capabilities.
Our study indicated that R-001 can use different types of estrogen as its sole carbon source for growth and metabolism, with final degradation rates above 90%.
Transcriptome analysis showed that 720 (E1), 983 (E2), and 845 (EE2) genes were significantly upregulated in the estrogen-treated group compared with the control group, and 270 differentially expressed genes (DEGs) were upregulated across all treatment groups.
These DEGs included ABC transporters; estrogen-degrading genes, including those that perform initial oxidation and dehydrogenation reactions and those that further degrade the resulting substrates into small molecules; and metabolism genes that complete the intracellular transformation and utilization of estrogen metabolites through biological processes such as amino acid metabolism, lipid metabolism, carbohydrate metabolism, and the tricarboxylic acid cycle.
In summary, the biodegradation of estrogens is coordinated by a metabolic network of estrogen-degrading enzymes, transporters, metabolic enzymes, and other coenzymes.
In this study, the metabolic mechanisms by which Rhodococcus equi R-001 degrades various estrogens were analyzed for the first time.
A new pollutant metabolism system is outlined, providing a starting point for the construction of engineered estrogen-degrading bacteria.
American Psychological Association (APA)
Tian, Kejian& Meng, Fanxing& Meng, Qi& Gao, Yan& Zhang, Lili& Wang, Le…[et al.]. 2020. The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001. International Journal of Genomics،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1171345
Modern Language Association (MLA)
Tian, Kejian…[et al.]. The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001. International Journal of Genomics No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1171345
American Medical Association (AMA)
Tian, Kejian& Meng, Fanxing& Meng, Qi& Gao, Yan& Zhang, Lili& Wang, Le…[et al.]. The Analysis of Estrogen-Degrading and Functional Metabolism Genes in Rhodococcus equi DSSKP-R-001. International Journal of Genomics. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1171345
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1171345