Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing
Joint Authors
Huang, Yufei
Meng, Jia
Wei, Zhen
Magalhães, João Pedro de
Rong, Rong
Lu, Zhiliang
Panneerdoss, Subbarayalu
Timilsina, Santosh
Zhu, Jingting
Mohammad, Tabrez A.
Rao, Manjeet K.
Chen, Yidong
Source
International Journal of Genomics
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-19, 19 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-13
Country of Publication
Egypt
No. of Pages
19
Main Subjects
Abstract EN
Background.
Compared with the well-studied 5-methylcytosine (m5C) in DNA, the role and topology of epitranscriptome m5C remain insufficiently characterized.
Results.
Through analyzing transcriptome-wide m5C distribution in human and mouse, we show that the m5C modification is significantly enriched at 5′ untranslated regions (5′UTRs) of mRNA in human and mouse.
With a comparative analysis of the mRNA and DNA methylome, we demonstrate that, like DNA methylation, transcriptome m5C methylation exhibits a strong clustering effect.
Surprisingly, an inverse correlation between mRNA and DNA m5C methylation is observed at CpG sites.
Further analysis reveals that RNA m5C methylation level is positively correlated with both RNA expression and RNA half-life.
We also observed that the methylation level of mitochondrial RNAs is significantly higher than RNAs transcribed from the nuclear genome.
Conclusions.
This study provides an in-depth topological characterization of transcriptome-wide m5C modification by associating RNA m5C methylation patterns with transcriptional expression, DNA methylations, RNA stabilities, and mitochondrial genome.
American Psychological Association (APA)
Wei, Zhen& Panneerdoss, Subbarayalu& Timilsina, Santosh& Zhu, Jingting& Mohammad, Tabrez A.& Lu, Zhiliang…[et al.]. 2018. Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing. International Journal of Genomics،Vol. 2018, no. 2018, pp.1-19.
https://search.emarefa.net/detail/BIM-1172669
Modern Language Association (MLA)
Wei, Zhen…[et al.]. Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing. International Journal of Genomics No. 2018 (2018), pp.1-19.
https://search.emarefa.net/detail/BIM-1172669
American Medical Association (AMA)
Wei, Zhen& Panneerdoss, Subbarayalu& Timilsina, Santosh& Zhu, Jingting& Mohammad, Tabrez A.& Lu, Zhiliang…[et al.]. Topological Characterization of Human and Mouse m5C Epitranscriptome Revealed by Bisulfite Sequencing. International Journal of Genomics. 2018. Vol. 2018, no. 2018, pp.1-19.
https://search.emarefa.net/detail/BIM-1172669
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1172669