The Lysine Demethylase KDM5B Regulates Islet Function and Glucose Homeostasis
Joint Authors
Poulsen, S. S.
Andreone, Luz
Mandrup-Poulsen, Thomas
Backe, Marie Balslev
Sankar, Aditya
Agger, Karl
Helin, Kristian
Madsen, Andreas Nygaard
Bysani, Madhusudhan
Bacos, Karl
Ling, Charlotte
Perone, Marcelo Javier
Holst, Birgitte
Jin, Chunyu
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-07-28
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Aims.
Posttranslational modifications of histones and transcription factors regulate gene expression and are implicated in beta-cell failure and diabetes.
We have recently shown that preserving H3K27 and H3K4 methylation using the lysine demethylase inhibitor GSK-J4 reduces cytokine-induced destruction of beta-cells and improves beta-cell function.
Here, we investigate the therapeutic potential of GSK-J4 to prevent diabetes development and examine the importance of H3K4 methylation for islet function.
Materials and Methods.
We used two mouse models of diabetes to investigate the therapeutic potential of GSK-J4.
To clarify the importance of H3K4 methylation, we characterized a mouse strain with knockout (KO) of the H3K4 demethylase KDM5B.
Results.
GSK-J4 administration failed to prevent the development of experimental diabetes induced by multiple low-dose streptozotocin or adoptive transfer of splenocytes from acutely diabetic NOD to NODscid mice.
KDM5B-KO mice were growth retarded with altered body composition, had low IGF-1 levels, and exhibited reduced insulin secretion.
Interestingly, despite secreting less insulin, KDM5B-KO mice were able to maintain normoglycemia following oral glucose tolerance test, likely via improved insulin sensitivity, as suggested by insulin tolerance testing and phosphorylation of proteins belonging to the insulin signaling pathway.
When challenged with high-fat diet, KDM5B-deficient mice displayed similar weight gain and insulin sensitivity as wild-type mice.
Conclusion.
Our results show a novel role of KDM5B in metabolism, as KDM5B-KO mice display growth retardation and improved insulin sensitivity.
American Psychological Association (APA)
Backe, Marie Balslev& Jin, Chunyu& Andreone, Luz& Sankar, Aditya& Agger, Karl& Helin, Kristian…[et al.]. 2019. The Lysine Demethylase KDM5B Regulates Islet Function and Glucose Homeostasis. Journal of Diabetes Research،Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1173037
Modern Language Association (MLA)
Backe, Marie Balslev…[et al.]. The Lysine Demethylase KDM5B Regulates Islet Function and Glucose Homeostasis. Journal of Diabetes Research No. 2019 (2019), pp.1-15.
https://search.emarefa.net/detail/BIM-1173037
American Medical Association (AMA)
Backe, Marie Balslev& Jin, Chunyu& Andreone, Luz& Sankar, Aditya& Agger, Karl& Helin, Kristian…[et al.]. The Lysine Demethylase KDM5B Regulates Islet Function and Glucose Homeostasis. Journal of Diabetes Research. 2019. Vol. 2019, no. 2019, pp.1-15.
https://search.emarefa.net/detail/BIM-1173037
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1173037