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Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus
Joint Authors
Bard, Gabriela C. V.
Taveira, Gabriel B.
Souza, Thaynã A. M.
Mello, Érica O.
Souza, Sávio B.
Ramos, Alessandro C.
Carvalho, André O.
Pereira, Lídia S.
Zottich, Umberto
Rodrigues, Rosana
Gomes, Valdirene M.
Source
International Journal of Microbiology
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The objective of the present study was to evaluate the antimicrobial activity of the Cc-LTP2 and Cc-GRP peptides isolated from Coffea canephora seeds and their possible synergistic activity with the azole drug fluconazole and characterize their mechanisms of action on cells of pathogenic fungi.
Cc-LTP2 and Cc-GRP alone or in combination with 20 µg/mL of fluconazole were evaluated for their antimicrobial activity on the fungus Fusarium solani, and the effects of these peptides on the permeability of membranes and the induction of oxidative stress were determined.
Our results show that these peptides at a concentration of 400 µg/mL combined with 20 µg/mL of fluconazole were able to inhibit the growth of the tested fungi, promote changes in their growth pattern, permeabilize the membrane, and induce reactive oxygen species (ROS).
Some of these results were also observed with the peptides alone or with fluconazole alone, suggesting that the peptides act synergistically, promoting the potentiation of antimicrobial action.
In this study, it was shown that Cc-LTP2 and Cc-GRP in combination with fluconazole were able to inhibit the growth of the fungus F.
solani, to promote permeabilization of its membrane, and to induce the production of ROS, suggesting a combinatorial activity between the peptides and fluconazole.
American Psychological Association (APA)
Bard, Gabriela C. V.& Taveira, Gabriel B.& Souza, Thaynã A. M.& Mello, Érica O.& Souza, Sávio B.& Ramos, Alessandro C.…[et al.]. 2018. Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus. International Journal of Microbiology،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1173402
Modern Language Association (MLA)
Bard, Gabriela C. V.…[et al.]. Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus. International Journal of Microbiology No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1173402
American Medical Association (AMA)
Bard, Gabriela C. V.& Taveira, Gabriel B.& Souza, Thaynã A. M.& Mello, Érica O.& Souza, Sávio B.& Ramos, Alessandro C.…[et al.]. Coffea canephora Peptides in Combinatorial Treatment with Fluconazole: Antimicrobial Activity against Phytopathogenic Fungus. International Journal of Microbiology. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1173402
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1173402