Urinary Exosomal MicroRNA Profiling in Incipient Type 2 Diabetic Kidney Disease
Joint Authors
Hu, Fang
Jia, Yijie
Xue, Yaoming
Xie, Yijun
Cuihua, Xie
Xue, Meng
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-09-05
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
Albuminuria is an early sign but not a strong predictor of diabetic kidney disease (DKD).
Owing to their high stability, urinary exosomal miRNAs can be useful predictors of the progression of early-stage DKD to renal failure; fluid biopsies are ideal for detecting abnormalities in these miRNAs.
The aim of this study was to identify novel differentially expressed miRNAs as urine biomarkers for type 2 DKD by comparing between patients of type 2 diabetes (T2D) with and without macroalbuminuria.
Methods.
Ten patients with T2D, including five who had no renal disease and five with macroalbuminuria (DKD G1-2A3), were selected for this study.
Exosome- (UExo-) derived miRNA profiles were used to identify candidate biomarkers, a subset of which was verified using quantitative reverse transcription PCR.
Results.
A total of 496 UExo-derived miRNA species were found to be differentially expressed (>2-fold) in patients with DKD, compared to those with T2D.
A validation analysis revealed that three miRNAs (miR-362-3p, miR-877-3p, and miR-150-5p) were upregulated and one (miR-15a-5p) was downregulated.
These miRNAs might regulate DKD through p53, mTOR, and AMPK pathways.
Conclusions.
In conclusion, UExo-derived miRNAs were altered in type 2 DKD.
MiR-362-3p, miR-877-3p, miR-150-5p, and miR-15a-5p might be novel biomarkers for incipient DKD.
American Psychological Association (APA)
Xie, Yijun& Jia, Yijie& Cuihua, Xie& Hu, Fang& Xue, Meng& Xue, Yaoming. 2017. Urinary Exosomal MicroRNA Profiling in Incipient Type 2 Diabetic Kidney Disease. Journal of Diabetes Research،Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1174644
Modern Language Association (MLA)
Xie, Yijun…[et al.]. Urinary Exosomal MicroRNA Profiling in Incipient Type 2 Diabetic Kidney Disease. Journal of Diabetes Research No. 2017 (2017), pp.1-10.
https://search.emarefa.net/detail/BIM-1174644
American Medical Association (AMA)
Xie, Yijun& Jia, Yijie& Cuihua, Xie& Hu, Fang& Xue, Meng& Xue, Yaoming. Urinary Exosomal MicroRNA Profiling in Incipient Type 2 Diabetic Kidney Disease. Journal of Diabetes Research. 2017. Vol. 2017, no. 2017, pp.1-10.
https://search.emarefa.net/detail/BIM-1174644
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174644