RP-HPLC and UV Spectrophotometric Analysis of Paracetamol, Ibuprofen, and Caffeine in Solid Pharmaceutical Dosage Forms by Derivative, Fourier, and Wavelet Transforms: A Comparison Study
Joint Authors
Truong Thi Thu, Huong
Dong Thi Ha, Ly
Nguyen Mai, Huong
Hoang, Vu Dang
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-02-08
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Different signal-transforming algorithms were applied for UV spectrophotometric analysis of paracetamol, ibuprofen, and caffeine in ternary mixtures.
Phosphate buffer pH 7.2 was used as the spectrophotometric solvent.
Severe overlapping spectra could be resolved into individual bands in the range of wavelengths 200–300 nm by using Savitzky–Golay smoothing and differentiation, trigonometric Fourier series, and mother wavelet functions (i.e., sym6, haar, coif3, and mexh).
To optimize spectral recoveries, the concentration of various types of divisors (single, double, and successive) was tested.
The developed spectrophotometric methods showed linearity over the ranges 20–40 mg/L for paracetamol, 12–32 mg/L for ibuprofen, and 1–3.5 mg/L for caffeine (R2 > 0.990).
They could be successfully applied to the assay and dissolution test of paracetamol, ibuprofen, and caffeine in their combined tablets and capsules, with accuracy (99.1–101.5% recovery) and precision (RSD < 2%).
For comparison, an isocratic RP-HPLC analysis was also developed and validated on an Agilent ZORBAX Eclipse XDB–C18 (150 × 4.6 mm, 5 µm) at an ambient temperature.
A mixture of methanol : phosphate buffer 0.01 M pH 3 (30 : 70 v/v) was used as the mobile phase delivered at 2 mL/min, and the effluent was monitored at 225 nm.
It was shown that spectrophotometric data were statistically comparable to HPLC p>0.05, suggesting possible interchange between UV spectrophotometric and HPLC methods for routine analysis of paracetamol, ibuprofen, and caffeine in their solid pharmaceutical dosage forms.
American Psychological Association (APA)
Hoang, Vu Dang& Truong Thi Thu, Huong& Dong Thi Ha, Ly& Nguyen Mai, Huong. 2020. RP-HPLC and UV Spectrophotometric Analysis of Paracetamol, Ibuprofen, and Caffeine in Solid Pharmaceutical Dosage Forms by Derivative, Fourier, and Wavelet Transforms: A Comparison Study. Journal of Analytical Methods in Chemistry،Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1174864
Modern Language Association (MLA)
Hoang, Vu Dang…[et al.]. RP-HPLC and UV Spectrophotometric Analysis of Paracetamol, Ibuprofen, and Caffeine in Solid Pharmaceutical Dosage Forms by Derivative, Fourier, and Wavelet Transforms: A Comparison Study. Journal of Analytical Methods in Chemistry No. 2020 (2020), pp.1-13.
https://search.emarefa.net/detail/BIM-1174864
American Medical Association (AMA)
Hoang, Vu Dang& Truong Thi Thu, Huong& Dong Thi Ha, Ly& Nguyen Mai, Huong. RP-HPLC and UV Spectrophotometric Analysis of Paracetamol, Ibuprofen, and Caffeine in Solid Pharmaceutical Dosage Forms by Derivative, Fourier, and Wavelet Transforms: A Comparison Study. Journal of Analytical Methods in Chemistry. 2020. Vol. 2020, no. 2020, pp.1-13.
https://search.emarefa.net/detail/BIM-1174864
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1174864