A Convenient and Sensitive LC-MSMS Method for Simultaneous Determination of Carbadox- and Olaquindox-Related Residues in Swine Muscle and Liver Tissues
Joint Authors
Huang, Lingli
Zhang, Heying
Qu, Wei
Tao, Yanfei
Chen, Dongmei
Xie, Shuyu
Liu, Zhenli
Pan, Yuanhu
Yuan, Zonghui
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-06-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
This paper presents a convenient and sensitive LC-MS/MS method for the simultaneous determination of carbadox and olaquindox residues, including desoxyolaquindox (DOLQ), desoxycarbadox (DCBX), quinoxaline-2-carboxylic acid (QCA), 3-methyl-quinoxaline-2-carboxylic acid (MQCA), and the glycine conjugates of QCA and MQCA (namely, QCA-glycine and MQCA-glycine, resp.) in swine muscle and liver tissues.
Tissue samples were extracted with 2% metaphosphoric acid in 20% methanol and cleaned up by solid-phase extraction (SPE) on a mixed-mode anion-exchange column (Oasis MAX).
Analysis was performed on a C18 column by detection with mass spectrometry in the multiple reaction monitoring (MRM) mode.
The limits of detection (LODs) of the six analytes were determined to be 0.01 μg·kg−1 to 0.25 μg·kg−1, and the limits of quantification (LOQs) were 0.02 μg·kg−1 to 0.5 μg·kg−1.
The total recoveries of the six analytes in all tissues were higher than 79.1% with the RSD% less than 9.2%.
The developed method can determine the real residue level of QCA and MQCA, whether they are present in free form or as glycine conjugates in tissues, together with the carcinogenic desoxy metabolites DCBX and DOLQ with high recovery.
Therefore, this method was suitable for routine analysis of residue control programmes and the residue depletion study of CBX and OLQ on swine.
American Psychological Association (APA)
Zhang, Heying& Qu, Wei& Tao, Yanfei& Chen, Dongmei& Xie, Shuyu& Huang, Lingli…[et al.]. 2018. A Convenient and Sensitive LC-MSMS Method for Simultaneous Determination of Carbadox- and Olaquindox-Related Residues in Swine Muscle and Liver Tissues. Journal of Analytical Methods in Chemistry،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1176293
Modern Language Association (MLA)
Zhang, Heying…[et al.]. A Convenient and Sensitive LC-MSMS Method for Simultaneous Determination of Carbadox- and Olaquindox-Related Residues in Swine Muscle and Liver Tissues. Journal of Analytical Methods in Chemistry No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1176293
American Medical Association (AMA)
Zhang, Heying& Qu, Wei& Tao, Yanfei& Chen, Dongmei& Xie, Shuyu& Huang, Lingli…[et al.]. A Convenient and Sensitive LC-MSMS Method for Simultaneous Determination of Carbadox- and Olaquindox-Related Residues in Swine Muscle and Liver Tissues. Journal of Analytical Methods in Chemistry. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1176293
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1176293