![](/images/graphics-bg.png)
Effect of Chitosan and Liposome Nanoparticles as Adjuvant Codelivery on the Immunoglobulin G Subclass Distribution in a Mouse Model
Joint Authors
Haryono, Agus
Salsabila, Korrie
Restu, Witta Kartika
Harmami, Sri Budi
Safari, Dodi
Source
Journal of Immunology Research
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-07-05
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
Background.
We investigate the immunogenic properties of chitosan and liposome nanoparticles as adjuvant codelivery against a commercial pneumococcal conjugate vaccine (PCV) in an animal model.
Methods.
The chitosan and liposome nanoparticles were prepared by ionic gelation and dry methods, respectively.
The PCV immunization was performed intradermally in the presence of adjuvants and booster injections which were given without an adjuvant.
The Quil-A® was used as a control adjuvant.
The ELISA was performed to measure the antibodies against pneumococcal type 14 polysaccharide (Pn14PS).
Results.
The level of total antibodies against Pn14PS antigen was no different between the mouse groups with or without adjuvant codelivery.
Codelivery of the PCV with chitosan nanoparticles as well as the Quil-A adjuvant elicited IgG1, IgG2a, IgG2b, and IgG3 antibodies.
Meanwhile, codelivery of liposome nanoparticles elicited mainly IgG1 antibodies against the Pn14PS.
Conclusions.
The chitosan and liposome nanoparticles as adjuvant codelivery were successfully synthesized.
These nanoparticles have different shapes in particle formation, liposome nanoparticle with their unilamellar shape and chitosan nanoparticles in large shape due to the aggregation of small-size particles.
Codelivery of chitosan nanoparticles has more effect on the IgG subclass antibody production than that of liposome nanoparticles in a mouse model.
American Psychological Association (APA)
Haryono, Agus& Salsabila, Korrie& Restu, Witta Kartika& Harmami, Sri Budi& Safari, Dodi. 2017. Effect of Chitosan and Liposome Nanoparticles as Adjuvant Codelivery on the Immunoglobulin G Subclass Distribution in a Mouse Model. Journal of Immunology Research،Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1182218
Modern Language Association (MLA)
Haryono, Agus…[et al.]. Effect of Chitosan and Liposome Nanoparticles as Adjuvant Codelivery on the Immunoglobulin G Subclass Distribution in a Mouse Model. Journal of Immunology Research No. 2017 (2017), pp.1-5.
https://search.emarefa.net/detail/BIM-1182218
American Medical Association (AMA)
Haryono, Agus& Salsabila, Korrie& Restu, Witta Kartika& Harmami, Sri Budi& Safari, Dodi. Effect of Chitosan and Liposome Nanoparticles as Adjuvant Codelivery on the Immunoglobulin G Subclass Distribution in a Mouse Model. Journal of Immunology Research. 2017. Vol. 2017, no. 2017, pp.1-5.
https://search.emarefa.net/detail/BIM-1182218
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182218