Physical Absorption of Folic Acid and Chitosan on Dihydroartemisinin-Loaded Poly-Lactic-Co-Glycolic Acid Nanoparticles via Electrostatic Interaction for Their Enhanced Uptake and Anticancer Effect
Joint Authors
Nguyen, Chien Ngoc
Tran, Bao Ngoc
Thi, Hoa Nguyen
Huu, Phong Pham
Thi, Huong Nguyen
Source
Issue
Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2019-06-13
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
In this study, dihydroartemisinin (DAR), an anticancer agent with low toxicity, was loaded into poly-lactic-co-glycolic acid (PLGA) nanoparticles.
The obtained PLGA cores were then coated with chitosan (CS) and/or folic acid (FA) by electrostatic interactions to enhance their anticancer and cellular uptake properties.
DAR-loaded PLGA nanoparticles were prepared by the solvent evaporation method.
CS and FA solutions at different ratios were dispersed concurrently into the PLGA suspension to facilitate electrostatic interactions and form nanosuspensions.
The physiochemical properties of nanoparticles such as average particle size (Z), polydispersity index (PDI), zeta potential (ZP), TEM image, X-ray diffraction, and encapsulation efficiency were determined.
We then determined the role of FA and CS coating on the nanoparticle surface in cytotoxicity, cellular uptake, and apoptosis.
We show that the resultant nanoparticles were spherical and uniform, with a coating layer containing FA and CS covering PLGA cores with a Z of 223.5±4.28 nm, PDI of 0.209±0.03, and ZP of 15.75±1.3 mV.
Both FA and CS improved the cytotoxicity of nanoparticles compared to free DAR and PLGA nanoparticles in HL-60 and KB cancer cell lines.
Further, FA enhanced the cellular uptake of nanoparticles to a greater extent than CS.
However, CS contributed more to apoptosis induction than FA.
American Psychological Association (APA)
Nguyen, Chien Ngoc& Tran, Bao Ngoc& Thi, Hoa Nguyen& Huu, Phong Pham& Thi, Huong Nguyen. 2019. Physical Absorption of Folic Acid and Chitosan on Dihydroartemisinin-Loaded Poly-Lactic-Co-Glycolic Acid Nanoparticles via Electrostatic Interaction for Their Enhanced Uptake and Anticancer Effect. Journal of Nanomaterials،Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1182830
Modern Language Association (MLA)
Nguyen, Chien Ngoc…[et al.]. Physical Absorption of Folic Acid and Chitosan on Dihydroartemisinin-Loaded Poly-Lactic-Co-Glycolic Acid Nanoparticles via Electrostatic Interaction for Their Enhanced Uptake and Anticancer Effect. Journal of Nanomaterials No. 2019 (2019), pp.1-14.
https://search.emarefa.net/detail/BIM-1182830
American Medical Association (AMA)
Nguyen, Chien Ngoc& Tran, Bao Ngoc& Thi, Hoa Nguyen& Huu, Phong Pham& Thi, Huong Nguyen. Physical Absorption of Folic Acid and Chitosan on Dihydroartemisinin-Loaded Poly-Lactic-Co-Glycolic Acid Nanoparticles via Electrostatic Interaction for Their Enhanced Uptake and Anticancer Effect. Journal of Nanomaterials. 2019. Vol. 2019, no. 2019, pp.1-14.
https://search.emarefa.net/detail/BIM-1182830
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1182830