Vitamin D Ameliorates Impaired Wound Healing in Streptozotocin-Induced Diabetic Mice by Suppressing Endoplasmic Reticulum Stress
Joint Authors
Das, Sushant Kumar
Yuan, Yi Feng
Li, Mao Quan
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-03-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
This study is designed to investigate whether vitamin D promotes diabetic wound healing and explore the potential mechanism which may be involved in the healing process.
Material and Methods.
Human umbilical vein endothelial cells (HUVECs) were treated with 200 μg/ml of advanced glycation end product-modified human serum albumin (AGE-HSA) and 250 mg/dl of glucose with vitamin D.
Cell viability was analyzed using the CCK-8 assay, and the apoptosis rate was measured using flow cytometry.
Endogenous markers of ER stress were quantified using Western blot and a real-time polymerase chain reaction.
Diabetic mice were treated with vitamin D (100 ng/kg per day) for 14 days.
The ulcer area and ulcerative histology were detected dynamically.
Results.
Vitamin D administration not only decreased the apoptosis rate but also increased cell viability.
Furthermore, the expression of endogenous markers of ER stress was downregulated as a result of vitamin D treatment.
Vitamin D supplementation significantly accelerated wound healing of diabetic mice and improved the healing quality.
Further studies showed that reduced ER stress was associated with the positive outcome.
Conclusion.
These results suggest that vitamin D may ameliorate impaired wound healing in diabetic mice by suppressing ER stress.
American Psychological Association (APA)
Yuan, Yi Feng& Das, Sushant Kumar& Li, Mao Quan. 2018. Vitamin D Ameliorates Impaired Wound Healing in Streptozotocin-Induced Diabetic Mice by Suppressing Endoplasmic Reticulum Stress. Journal of Diabetes Research،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1183303
Modern Language Association (MLA)
Yuan, Yi Feng…[et al.]. Vitamin D Ameliorates Impaired Wound Healing in Streptozotocin-Induced Diabetic Mice by Suppressing Endoplasmic Reticulum Stress. Journal of Diabetes Research No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1183303
American Medical Association (AMA)
Yuan, Yi Feng& Das, Sushant Kumar& Li, Mao Quan. Vitamin D Ameliorates Impaired Wound Healing in Streptozotocin-Induced Diabetic Mice by Suppressing Endoplasmic Reticulum Stress. Journal of Diabetes Research. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1183303
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183303