Study of the Dynamic Uptake of Free Drug and Nanostructures for Drug Delivery Based on Bioluminescence Measurements
Joint Authors
Fang, Zhongjian
Xu, Houchao
Ji, Xiangjun
Liu, Congbiao
Wang, Kai
Qian, Xiaoqing
Zhou, Wencong
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-02-23
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The past two decades have witnessed the great growth of the development of novel drug carriers.
However, the releasing dynamics of drug from drug carriers in vivo and the interactions between cells and drug carriers remain unclear.
In this paper, liposomes were prepared to encapsulate D-luciferin, which was the substrate of luciferase and served as a model drug.
Based on the theoretical calculation of active loading, methods of preparation for liposomes were optimized.
Only when D-luciferin was released from liposomes or taken in by the cells could bioluminescence be produced under the catalysis of luciferase.
Models of multicellular tumor spheroid (MCTS) were built with 4T1-luc cells that expressed luciferase stably.
The kinetic processes of uptake and distribution of free drugs and liposomal drugs were determined with models of cell suspension, monolayer cells, MCTS, and tumor-bearing nude mice.
The technology platform has been demonstrated to be effective for the study of the distribution and kinetic profiles of various liposomes as drug delivery systems.
American Psychological Association (APA)
Fang, Zhongjian& Xu, Houchao& Ji, Xiangjun& Liu, Congbiao& Wang, Kai& Qian, Xiaoqing…[et al.]. 2017. Study of the Dynamic Uptake of Free Drug and Nanostructures for Drug Delivery Based on Bioluminescence Measurements. Journal of Nanomaterials،Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1183694
Modern Language Association (MLA)
Fang, Zhongjian…[et al.]. Study of the Dynamic Uptake of Free Drug and Nanostructures for Drug Delivery Based on Bioluminescence Measurements. Journal of Nanomaterials No. 2017 (2017), pp.1-12.
https://search.emarefa.net/detail/BIM-1183694
American Medical Association (AMA)
Fang, Zhongjian& Xu, Houchao& Ji, Xiangjun& Liu, Congbiao& Wang, Kai& Qian, Xiaoqing…[et al.]. Study of the Dynamic Uptake of Free Drug and Nanostructures for Drug Delivery Based on Bioluminescence Measurements. Journal of Nanomaterials. 2017. Vol. 2017, no. 2017, pp.1-12.
https://search.emarefa.net/detail/BIM-1183694
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183694