Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy
Joint Authors
Guillén-Gómez, Elena
Bardají-de-Quixano, Beatriz
Ferrer, Sílvia
Brotons, Carlos
Knepper, Mark A.
Carrascal, Montserrat
Abian, Joaquin
Mas, José M.
Calero, Francesca
Ballarín, José A.
Fernández-Llama, Patricia
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-04-29
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Urinary proteome was analyzed and quantified by tandem mass tag (TMT) labeling followed by bioinformatics analysis to study diabetic nephropathy (DN) pathophysiology and to identify biomarkers of a clinical outcome.
We included type 2 diabetic normotensive non-obese males with (n=9) and without (n=11) incipient DN (microalbuminuria).
Sample collection included blood and urine at baseline (control and DN basal) and, in DN patients, after 3 months of losartan treatment (DN treated).
Urinary proteome analysis identified 166 differentially abundant proteins between controls and DN patients, 27 comparing DN-treated and DN-basal patients, and 182 between DN-treated patients and controls.
The mathematical modeling analysis predicted 80 key proteins involved in DN pathophysiology and 15 in losartan effect, a total of 95 proteins.
Out of these 95, 7 are involved in both processes.
VCAM-1 and neprilysin stand out of these 7 for being differentially expressed in the urinary proteome.
We observed an increase of VCAM-1 urine levels in DN-basal patients compared to diabetic controls and an increase of urinary neprilysin in DN-treated patients with persistent albuminuria; the latter was confirmed by ELISA.
Our results point to neprilysin and VCAM-1 as potential candidates in DN pathology and treatment.
American Psychological Association (APA)
Guillén-Gómez, Elena& Bardají-de-Quixano, Beatriz& Ferrer, Sílvia& Brotons, Carlos& Knepper, Mark A.& Carrascal, Montserrat…[et al.]. 2018. Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy. Journal of Diabetes Research،Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1183751
Modern Language Association (MLA)
Guillén-Gómez, Elena…[et al.]. Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy. Journal of Diabetes Research No. 2018 (2018), pp.1-12.
https://search.emarefa.net/detail/BIM-1183751
American Medical Association (AMA)
Guillén-Gómez, Elena& Bardají-de-Quixano, Beatriz& Ferrer, Sílvia& Brotons, Carlos& Knepper, Mark A.& Carrascal, Montserrat…[et al.]. Urinary Proteome Analysis Identified Neprilysin and VCAM as Proteins Involved in Diabetic Nephropathy. Journal of Diabetes Research. 2018. Vol. 2018, no. 2018, pp.1-12.
https://search.emarefa.net/detail/BIM-1183751
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1183751