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Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding
Joint Authors
Sacchetti, Lucia
Nardelli, Carmela
Ferrigno, Maddalena
Buono, Pasqualina
Dodaro, Concetta
Carlomagno, Nicola
Pilone, Vincenzo
Iaffaldano, Laura
Forestieri, Pietro
Salvatore, Francesco
Labruna, Giuseppe
Source
Issue
Vol. 2017, Issue 2017 (31 Dec. 2017), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2017-03-13
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Background.
Laparoscopic adjustable gastric banding (LAGB) results in significant lasting weight loss and improved metabolism in obese patients.
To evaluate whether epigenetic factors could concur to these benefits, we investigated the subcutaneous adipose tissue (SAT) microRNA (miRNA) profile before (T0) and three years (T1) after LAGB in three morbidly obese women.
Case Reports.
SAT miRNA profiling, evaluated by TaqMan Array, showed four downexpressed (miR-519d, miR-299-5p, miR-212, and miR-671-3p) and two upexpressed (miR-370 and miR-487a) miRNAs at T1 versus T0.
Bioinformatics predicted that these miRNAs regulate genes belonging to pathways associated with the cytoskeleton, inflammation, and metabolism.
Western blot analysis showed that PPAR-alpha, which is the target gene of miR-519d, increased after LAGB, thereby suggesting an improvement in SAT lipid metabolism.
Accordingly, the number and diameter of adipocytes were significantly higher and lower, respectively, at T1 versus T0.
Bioinformatics predicted that the decreased levels of miR-212, miR-299-5p, and miR-671-3p at T1 concur in reducing SAT inflammation.
Conclusion.
We show that the miRNA profile changes after LAGB.
This finding, although obtained in only three cases, suggests that this epigenetic mechanism, by regulating the expression of genes involved in inflammation and lipid metabolism, could concur to improve SAT functionality in postoperative obese patients.
American Psychological Association (APA)
Nardelli, Carmela& Iaffaldano, Laura& Pilone, Vincenzo& Labruna, Giuseppe& Ferrigno, Maddalena& Carlomagno, Nicola…[et al.]. 2017. Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding. Journal of Obesity،Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1184318
Modern Language Association (MLA)
Nardelli, Carmela…[et al.]. Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding. Journal of Obesity No. 2017 (2017), pp.1-6.
https://search.emarefa.net/detail/BIM-1184318
American Medical Association (AMA)
Nardelli, Carmela& Iaffaldano, Laura& Pilone, Vincenzo& Labruna, Giuseppe& Ferrigno, Maddalena& Carlomagno, Nicola…[et al.]. Changes in the MicroRNA Profile Observed in the Subcutaneous Adipose Tissue of Obese Patients after Laparoscopic Adjustable Gastric Banding. Journal of Obesity. 2017. Vol. 2017, no. 2017, pp.1-6.
https://search.emarefa.net/detail/BIM-1184318
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1184318