Determination of Vitamins D2 and D3 in Edible Fungus by Reversed-Phase Two-Dimensional Liquid Chromatography
Joint Authors
Huang, Bai-Fen
Pan, Xiao-Dong
Zhang, Jing-Shun
Xu, Jiao-Jiao
Cai, Zeng-Xuan
Source
Issue
Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2020-09-22
Country of Publication
Egypt
No. of Pages
6
Abstract EN
Vitamin D is the name given to a series of compounds with antirachitic activity.
In this study, we developed a method for quantification of the common Vitamin D, ergocalciferol (vitamin D2), and cholecalciferol (vitamin D3), in edible fungus by reversed-phase two-dimensional liquid chromatography.
The sample was saponified by KOH ethanol solution and extracted with ethyl acetate and n-hexane.
After reconcentration, the sample was directly injected for instrumental analysis.
The results showed that the spiking recoveries of vitamin D2 and vitamin D3 were 80.4–93.8% and 82.3–92.0%, respectively.
The relative standard deviations (RSDs) were all less than 5%.
Comparing to the results of isotope dilution liquid chromatography tandem mass spectrometry, there were no significant differences (P>0.05).
The method was applied for testing vitamin D2 and D3 in 9 kinds of edible mushrooms.
It showed that vitamin D3 was not detected.
The content of vitamin D2 ranged from 0.11 μg/100 g to 123 μg/100 g.
The content of vitamin D2 in dried mushroom was the highest, while that in fresh mushroom was the lowest.
The limit of quantification was 0.025 μg/100 g.
American Psychological Association (APA)
Huang, Bai-Fen& Pan, Xiao-Dong& Zhang, Jing-Shun& Xu, Jiao-Jiao& Cai, Zeng-Xuan. 2020. Determination of Vitamins D2 and D3 in Edible Fungus by Reversed-Phase Two-Dimensional Liquid Chromatography. Journal of Food Quality،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1184966
Modern Language Association (MLA)
Huang, Bai-Fen…[et al.]. Determination of Vitamins D2 and D3 in Edible Fungus by Reversed-Phase Two-Dimensional Liquid Chromatography. Journal of Food Quality No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1184966
American Medical Association (AMA)
Huang, Bai-Fen& Pan, Xiao-Dong& Zhang, Jing-Shun& Xu, Jiao-Jiao& Cai, Zeng-Xuan. Determination of Vitamins D2 and D3 in Edible Fungus by Reversed-Phase Two-Dimensional Liquid Chromatography. Journal of Food Quality. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1184966
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1184966