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Methods for Extraction of Muscle Proteins from Meat and Fish Using Denaturing and Nondenaturing Solutions
Joint Authors
Marino, Rosaria
Malva, Antonella della
Albenzio, Marzia
Santillo, Antonella
Russo, Donatella
Figliola, Lucia
Caroprese, Mariangela
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-28
Country of Publication
Egypt
No. of Pages
9
Abstract EN
The objective of the present study was to test two extraction methods including solutions with different ionic strengths on the extraction and separation of the myofibrillar proteins from meat and fish muscles of different species.
Meat samples from longissimus thoracis muscle of beef and lamb, pectoralis major muscle of chicken, and dorsal white muscle of fish from sole (Solea solea), European hake (Merluccius merluccius), and sea bass (Dicentrarchus labrax) were analyzed.
The extraction method using nondenaturing solution led to a major extraction of high molecular-weight proteins as myosin heavy chain, α-actinin, and desmin; on the contrary, the denaturing method provided a good protein extractability of proteins and fragments with low molecular-weight as actin, troponin-T, tropomyosin, and myosin light chain 1 and 2 proteins for the most meat and fish samples.
The nondenaturing extraction method showed several advantages resulting in time and labour saving and in minimizing the use of toxic and polluting agents.
American Psychological Association (APA)
Malva, Antonella della& Albenzio, Marzia& Santillo, Antonella& Russo, Donatella& Figliola, Lucia& Caroprese, Mariangela…[et al.]. 2018. Methods for Extraction of Muscle Proteins from Meat and Fish Using Denaturing and Nondenaturing Solutions. Journal of Food Quality،Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1185529
Modern Language Association (MLA)
Malva, Antonella della…[et al.]. Methods for Extraction of Muscle Proteins from Meat and Fish Using Denaturing and Nondenaturing Solutions. Journal of Food Quality No. 2018 (2018), pp.1-9.
https://search.emarefa.net/detail/BIM-1185529
American Medical Association (AMA)
Malva, Antonella della& Albenzio, Marzia& Santillo, Antonella& Russo, Donatella& Figliola, Lucia& Caroprese, Mariangela…[et al.]. Methods for Extraction of Muscle Proteins from Meat and Fish Using Denaturing and Nondenaturing Solutions. Journal of Food Quality. 2018. Vol. 2018, no. 2018, pp.1-9.
https://search.emarefa.net/detail/BIM-1185529
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1185529