Large-Scale Screening in General Population Children for Celiac Disease with a Multiplex Electrochemiluminescence (ECL)‎ Assay

Joint Authors

Rewers, Marian
Miao, Dongmei
Waugh, Kathleen
Gu, Yong
He, Ling
Jia, Xiaofan
Geno, Cristy
Yu, Liping
Liu, Edwin

Source

Journal of Immunology Research

Issue

Vol. 2020, Issue 2020 (31 Dec. 2020), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2020-12-24

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Biology

Abstract EN

Background.

Autoimmunity Screening for Kids (ASK) study was launched to screen general population children for type 1 diabetes (T1D) and celiac disease (CD).

Methods.

A total of 23,319 children from general population were screened.

A high throughput multiplex electrochemiluminescence (ECL) assay to screen multiautoantibodies in a single well was applied, parallel with a standard radiobinding assay (RBA).

All children with any positive autoantibodies in screening were revisited within one month for confirmation and followed every 6 months.

Results.

Among 23,319 children, 2.6% (606/23,319) of children were tested positive for TGA.

Multiplex ECL assay detected more TGA (584/23,319) in the initial screening than RBA (490/23,319, p=0.004) and was able to detect TGA earlier than RBA in a subset of children by 0.8 to 34.8 months.

Prevalence of TGA by either ECL or RBA in children with islet autoantibodies was found significantly higher than overall prevalence in general population screened.

Conclusions.

A multiplex ECL assay was more sensitive than standard RBA by identifying more TGA positivity and detecting TGA earlier in general population screening.

It also provides a high efficient tool with its unique advantage of multiplexing measurements to screen for multiple autoimmune diseases simultaneously in general population.

American Psychological Association (APA)

He, Ling& Jia, Xiaofan& Gu, Yong& Miao, Dongmei& Waugh, Kathleen& Geno, Cristy…[et al.]. 2020. Large-Scale Screening in General Population Children for Celiac Disease with a Multiplex Electrochemiluminescence (ECL) Assay. Journal of Immunology Research،Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1187644

Modern Language Association (MLA)

He, Ling…[et al.]. Large-Scale Screening in General Population Children for Celiac Disease with a Multiplex Electrochemiluminescence (ECL) Assay. Journal of Immunology Research No. 2020 (2020), pp.1-6.
https://search.emarefa.net/detail/BIM-1187644

American Medical Association (AMA)

He, Ling& Jia, Xiaofan& Gu, Yong& Miao, Dongmei& Waugh, Kathleen& Geno, Cristy…[et al.]. Large-Scale Screening in General Population Children for Celiac Disease with a Multiplex Electrochemiluminescence (ECL) Assay. Journal of Immunology Research. 2020. Vol. 2020, no. 2020, pp.1-6.
https://search.emarefa.net/detail/BIM-1187644

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1187644